Content Determination and Evaluation of Sterilization Method in Baoji Pills by HPLC

WEN Jin-lian; GUO Li-bing; ZHOU Qing; TANG Rui; LIANG Ze-ming; WANG Jing-fang; ZHU Sheng-shan

doi:10.13422/j.cnki.syfjx.2011.23.015

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Content Determination and Evaluation of Sterilization Method in Baoji Pills by HPLC

更新时间：2024-09-23

- Content Determination and Evaluation of Sterilization Method in Baoji Pills by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 23, Pages: 1-4(2011)
- 作者机构：
  
  广东药学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.23.015
  CLC： R286
- Published：2011
- 稿件说明：
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[1]温金莲,郭丽冰,周清,唐睿,梁泽铭,王景芳,朱盛山.保济丸中葛根素的含量测定及灭菌方法评价[J].中国实验方剂学杂志,2011,17(23):1-4.

WEN Jin-lian, GUO Li-bing, ZHOU Qing, et al. Content Determination and Evaluation of Sterilization Method in Baoji Pills by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(23): 1-4.
[1]温金莲,郭丽冰,周清,唐睿,梁泽铭,王景芳,朱盛山.保济丸中葛根素的含量测定及灭菌方法评价[J].中国实验方剂学杂志,2011,17(23):1-4. DOI： 10.13422/j.cnki.syfjx.2011.23.015.

WEN Jin-lian, GUO Li-bing, ZHOU Qing, et al. Content Determination and Evaluation of Sterilization Method in Baoji Pills by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(23): 1-4. DOI： 10.13422/j.cnki.syfjx.2011.23.015.

摘要

目的:建立保济丸中葛根素含量的测定方法

评价几种灭菌方法的优劣。方法:以甲醇-水（30∶70）为流动相

流速1.0 mL.min-1

检测波长250 nm

柱温30℃

用RP-HPLC测定经各种灭菌方法处理的保济丸中葛根素的含量与未经灭菌处理的保济丸中葛根素的含量。结果:葛根素在4.212～84.24 mg.L-1呈良好的线性关系（r=0.999 9）

平均回收率98.18%

RSD 0.44%。湿热蒸汽灭菌方法在4种灭菌方法中属最优法。结论:定量测定方法简便、快速、灵敏、结果准确、重复性好

可作为保济丸的质量控制标准。

Abstract

Objective:To establish content determination method of puerarin from Baoji pills

evaluated superiority and inferior of several sterilization method.Method: In this method

methanol-water（30∶70） at flow rate of 1.0 mL·min-1 as mobile phase

detection wavelength was 250 nm at 30 ℃.Determined the content of puerarin in Baoji pills by RP-HPLC

which handled by various sterilization methods or without handling by any sterilization method.Result: Puerarin showed a good linear relationship at range of 4.212-84.24 mg·L-1（r=0.999 9）.Average recovery was 98.18%

and RSD 0.44%.Heat steam sterilization method was the best method in this four methods.Conclusion: It was simple

rapid

high sensitivity and accurate with good repeatability and could be used for quality control in Baoji pills.

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Related Author

WEN Jin-lian

GUO Li-bing

ZHOU Qing

TANG Rui

LIANG Ze-ming

WANG Jing-fang

ZHU Sheng-shan

LI Xue-qing

Related Institution

Chengde Jingfukang Pharmaceutical Group Co.,Ltd.

Hebei Provincial Engineering Technology Research Center for New Pharmaceutical Excipients in Traditional Chinese Medicine

Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences and Peking Union Medical College

Jilin Agricultural University

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

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