Objective:To quickly and effectively discriminate Radix Paeoniae Rubra originates from Paeonia lactiflora and Paeonia veitchii

and evaluate the quality of them by HPLC fingerprint.Method:HPLC fingerprint was adopted to analyze 12 batches of P.lactiflora and 29 batches of P.veitchii that from different fields and picking time

and setting the profile of reference crude drug of P.lactiflora and P.veitchii Lynch as a control.The similarity was evaluated according to the software-’Herb Fingerprint Similarity Assessment System’（2004A）

and established Fisher’s linear discriminant functions were astablished by discriminant analysis.Result: The similarity index of reference crude drug of P.lactiflora and P.veitchii is about 0.7

lower than 0.9.The similarity index of 12 batches P.lactiflora and 29 batches P.veitchii with their relative reference crude drug is both above 0.9.The Fisher’s linear discriminant functions was as follows: Y（P.lactiflora）=139.797X1+21.961X2+30.927X3-14.796

Y（P.veitchii）=45.688 X1-10.867X2+69.898X3-13.440（The independentsX1

X2

X3were respectively stand for relative area of catechin

albiflorin and benzoic acid with paeoniflorin area as reference）.Conclusion:Combining the chemometric.Such as similarity evalution or discriminant analysis

we could quickly and effectively discriminate Radix Paeoniae Rubra by HPLC fingerprint.Component similarity and differentiation were existing between P.lactiflora and P.veitchii

and P.veitchii was more complex.Species differentiation was the major factor of different constituents between them

fields and picking time were not important.In order to instruct application in clinic safely

it was worth establishing normative quality standard of Radix Paeoniae Rubra by relative research and experiment.