Simultaneous Determination of Emodin,chrysophanol and Physcion In Lidanpaishi Tablet by HPLC

LI Xin-jian1; FANG Cui-fen2; ZHU Ming2

doi:10.13422/j.cnki.syfjx.2011.23.023

您当前的位置：

首页 >

文章列表页 >

Simultaneous Determination of Emodin,chrysophanol and Physcion In Lidanpaishi Tablet by HPLC

更新时间：2024-09-23

- Simultaneous Determination of Emodin,chrysophanol and Physcion In Lidanpaishi Tablet by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 23, Pages: 82-84(2011)
- 作者机构：
  
  1. 浙江中医药大学
  2. 浙江省食品药品检验所
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.23.023
  CLC： R286.0
- Published：2011
- 稿件说明：
移动端阅览
[1]李鑫健,方翠芬,祝明.HPLC法同时测定利胆排石片中大黄素、大黄酚及大黄素甲醚[J].中国实验方剂学杂志,2011,17(23):82-84.

LI Xin-jian1, FANG Cui-fen2, ZHU Ming2. Simultaneous Determination of Emodin,chrysophanol and Physcion In Lidanpaishi Tablet by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(23): 82-84.
[1]李鑫健,方翠芬,祝明.HPLC法同时测定利胆排石片中大黄素、大黄酚及大黄素甲醚[J].中国实验方剂学杂志,2011,17(23):82-84. DOI： 10.13422/j.cnki.syfjx.2011.23.023.

LI Xin-jian1, FANG Cui-fen2, ZHU Ming2. Simultaneous Determination of Emodin,chrysophanol and Physcion In Lidanpaishi Tablet by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(23): 82-84. DOI： 10.13422/j.cnki.syfjx.2011.23.023.

摘要

目的:建立高效液相色谱法同时测定利胆排石片中大黄素、大黄酚及大黄素甲醚含量的方法。方法:采用高效液相色谱法

Agilent SB-C18（4.6 mm×250 mm

5μm）色谱柱

以乙腈-甲醇-0.1%磷酸（42∶23∶35）为流动相

流速1.0 mL.min-1

检测波长254 nm。结果:大黄素、大黄酚和大黄素甲醚分别在3.4×10-3～1.14μg

5.3×10-3～1.06μg

1.9×10-3～0.49μg线性关系良好

r分别为0.999 9

0.999 9

0.999 6;平均加样回收率分别为101.1%

100.5%

99.3%

RSD（n=6）分别为1.1%

0.9%

1.1%。结论:方法简便快捷

结果准确、可靠

重复性好

可作为利胆排石片质量控制方法之一。

Abstract

Objective:To establish an HPLC method for the content determination of emodin

chrysophanol and physcion in Lidanpaishi tablet.Method:The analysis was carried out with Agilent SB-C18（4.6 mm×250 mm

5 μm）column and the mobile phase consisted of Acetontrile-Methanal-0.1%phosphoric acid solution（42∶35∶23）at a flow of 1.0 mL·min-1.The detection wavelength was 254 nm.Result:The calibration curve was linear within the range of 3.4×10-3-1.14 μg（r=0.999 9）

5.3×10-3-1.06 μg（r=0.999 8） and 1.9×10-3-0.49 μg（r=0.999 2） for emodin

chrysophanol and physcion

respectively.The average recoveries were 101.1%（RSD 1.1%）

100.5%（RSD 0.9%） and 99.3%（RSD 1.1%） for emodin

chrysophanol and physcion

respectively.Conclusion:The method is simple

accurate

reproducible and prcise

can be used for the quality of Lidanpaishi tablets.

关键词

Keywords

references

Views

156

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Quantitative Determination of Emodin and Chrysophanol in Tuizhang Hydrogel by HPLC

Determination of Aloeemodin,Rhein,Emodin and Chrysophanol in Qingyi Tablets by HPLC

Determination of Emodin,Chrysophanol in Yankening Tablets by HPLC

Related Author

LI Xin-jian

FANG Cui-fen

ZHU Ming

WANG Guo-hua

LI Hui

NIE Qi-xia

ZANG chen

ZHANG Bao-xian

Related Institution

Xuzhou Medical Colledge

Tian Jin Nan Kai Hospital

Key Laboratory of Digital Quality Evaluation Technology of Chinese Materia Medica, State Administration of Traditional Chinese Medicine(TCM), Engineering Technology Research Center for Chinese Materia Medica Quality of Universities in Guangdong Province, School of TCM, Guangdong Pharmaceutical University

School of Pharmacy,Guiyang Medical College

The Second Affiliated Hospital of Air Force Medical University

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰