Objective:To investigate the protective effects and its antioxidant mechanism of tetramethylpyrazine on renal injury induced by high-fat diet and streptozotocin（STZ）.Method:Seventy rats were randomly divided into normal control group（14 rats） and model group.All model rats were fed by high-fat diet for 4 weeks and then injected with STZ（40 mg·kg-1

ip）.Blood glucose levels were measured 72 h after STZ injection using a one-touch glucometer.Rats with fasting blood glucose level above 16.67 mmol·L-1 were randomly divided into four groups: model control

metformin（250 mg·kg-1）and tetramethylpyrazine（80

160 mg·kg-1） groups.All rats were treated for 8 weeks.At the end of experiment

urine was collected from the rats for 24 h to measure the urinary volume and protein.And then blood was collected to detect the changes of creatinine（Cr） and blood urea nitrogen（BUN）

contents of glycated hemoglobin（HbAlc） and advanced glycation end products（AGEs）.Contents of AGEs and malondialdehyde（MDA）

activity of superoxide dismutase（SOD） and Na+-K+-ATP enzyme in kidney tissues were tested respectively.Result: Compared with normal control

the levels of SCr

BUN were significantly increased in the models（P<0.01）

content of MDA increased

while activity of SOD and Na+-K+-ATP enzyme decreased significantly（P<0.01） in kidney tissues

and contents of HbAlc

concentrations of AGEs in serum and kidney were increased markedly.Treated with Tetramethylpyrazine（80

160 mg·kg-1） for 8 weeks

the levels of SCr and BUN in blood were decreased（P<0.05）;and the MDA content in kidney tissues was reduced

activities of SOD and Na+-K+-ATP enzyme were also enhanced respectively（P<0.01

P<0.05）

and contents of HbAlc

concentrations of AGEs in serum and kidney were decreased markedly.Conclusion:Tetramethylpyrazine appears protective effect for renal injury rats induced by high-fat diet and STZ

and its mechanism may be concerned with increasing the antioxidative ability.