LIANG Hui, ZOU Sheng-qin. Simultaneous Determination of Polygonin and Resveratrol in Different Age Root of Polygonum cuspidatum by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(23): 62-64.
LIANG Hui, ZOU Sheng-qin. Simultaneous Determination of Polygonin and Resveratrol in Different Age Root of Polygonum cuspidatum by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(23): 62-64. DOI: 10.13422/j.cnki.syfjx.2011.23.050.
Simultaneous Determination of Polygonin and Resveratrol in Different Age Root of Polygonum cuspidatum by RP-HPLC
摘要
目的:建立反相高效液相色谱法同时测定不同根龄虎杖根中虎杖苷和白藜芦醇含量。方法:采用Kromasil C18(4.6 mm×250 mm
Objective:To develop a reserved-phase HPLC method for the content determination of polygonin and resveratrol in different age root of Polygonum cuspidatum.Method: The analysis was carried out with Kromasil C18(4.6 mm×250 mm
5 μm) column and the mobile phase consisted of acetonitrile-1.0% phosphoric acid aqueous solution(50∶50) at a flow rate of 0.8 mL·min-1.The detection wavelength was 319 nm and the column temperature was set at 30 ℃.Result: The calibration curves were linear within the range of 0.716-7.160 μg(r=0.999 6) for polygonin and 0.740-7.400 μg(r=0.999 8) for resveratrol.The average recoveries were 99.7% for erbascoside and 98.5% for resveratrol.Conclusion: The method is simple
accurate
reproducible and precise
and applicable for the determination of polygonin and resveratrol in different age root of Polygonum cuspidatum.
Simultaneous Quantitative Analysis of Polygonin, Resveratrol, Archen and Emodin Monomethyl Ether in Polygoni Cuspidati Rhizoma et Radix by Multi-Components Assay and Single Marker
Molecular Mechanism of Resveratrol Against NAFLD Based on Network Pharmacology
Pre-column Derivatization RP-HPLC for Simultaneous Determination of 17 Amino Acids in Cynomorii Herba from Different Producing Areas and Its Multivariate Statistical Analysis
Preparation of Magnetic Solid Lipid Nanoparticles Co-loaded Quercetin and Resveratrol and Evaluation of Its Antitumor Effect
Related Author
LIANG Hui
ZOU Sheng-qin
FAN Ling
YAN Dong
LI Shuang
MA Xing-miao
QIAN Yu-liang
LIU Zhi-hui
Related Institution
School of Pharmacy,Guangdong Pharmaceutical University
School of Life Sciences and Biopharmaceutics,Guangdong Pharmaceutical University
School of Basic Medical Sciences, Guangzhou University of Chinese Medicine
College of Pharmacy, Gansu University of Chinese Medicine
Gansu Province Key Laboratory of Pharmacology and Toxicology of Traditional Chinese Medicine