JU Cheng-guo, WANG Wei, GAO Hui, et al. Determination of Costunolide and Dehydrocostus Lactone in Muxiangbinglang Pill by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(23): 74-76.
JU Cheng-guo, WANG Wei, GAO Hui, et al. Determination of Costunolide and Dehydrocostus Lactone in Muxiangbinglang Pill by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(23): 74-76. DOI: 10.13422/j.cnki.syfjx.2011.23.054.
Determination of Costunolide and Dehydrocostus Lactone in Muxiangbinglang Pill by HPLC
Objective:To develop a HPLC method for the determination of costunolide and dehydrocostus lactone in Muxiangbinglang pill.Method: An Agilent C18 column(4.6 mm×250 mm
5 μm) was used
the mobile phase was methanol-water(65∶35)
the detection wavelength was at 225 nm
the column temperature was at 30 ℃ and the flow rate was 1.0 mL·min-1.Result:Costunolide showed a good linear relationship in the range of was 0.046 4-0.371 2 μg(r=0.999 6)
the average recovery was 99.0%
RSD 0.86%(n=6).Dehydrocostus lactone showed a good linear relationship in the range of was 0.045 2-0.361 6 μg(r=0.999 5)
the average recovery was 99.2%
RSD 0.23%(n=6).Conclusion: The method is accurate and reliable
it can be used for the quality control of Muxiangbinglang pill.