Determination of Oleanolic Acid and Ursolic Acid in Halenia elliptica by HPLC

FENG Jing1; WANG Li-li2; ZHANG Tie-jun2

doi:10.13422/j.cnki.syfjx.2011.23.056

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Determination of Oleanolic Acid and Ursolic Acid in Halenia elliptica by HPLC

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- Determination of Oleanolic Acid and Ursolic Acid in Halenia elliptica by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 23, Pages: 79-81(2011)
- 作者机构：
  
  1. 天津中医药大学
  2. 天津药物研究院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.23.056
  CLC： R284.1
- Published：2011
- 稿件说明：
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[1]冯京,王丽莉,张铁军.HPLC同时测定藏药花锚中齐墩果酸和熊果酸含量[J].中国实验方剂学杂志,2011,17(23):79-81.

FENG Jing1, WANG Li-li2, ZHANG Tie-jun2. Determination of Oleanolic Acid and Ursolic Acid in Halenia elliptica by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(23): 79-81.
[1]冯京,王丽莉,张铁军.HPLC同时测定藏药花锚中齐墩果酸和熊果酸含量[J].中国实验方剂学杂志,2011,17(23):79-81. DOI： 10.13422/j.cnki.syfjx.2011.23.056.

FENG Jing1, WANG Li-li2, ZHANG Tie-jun2. Determination of Oleanolic Acid and Ursolic Acid in Halenia elliptica by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(23): 79-81. DOI： 10.13422/j.cnki.syfjx.2011.23.056.

摘要

目的:建立同时测定藏药花锚中齐墩果酸和熊果酸含量的测定方法。方法:采用高效液相色谱法

流动相甲醇-0.5%磷酸水（85:15）

柱温20℃

流速0.8 mL.min-1

检测波长210 nm。结果:齐墩果酸和熊果酸在0.01～0.05

0.02～0.1 g.L-1呈良好的线性关系

加样回收率分别为101.17%

101.79%

RSD分别为1.90%

1.65%。结论:该法简便、快捷

结果准确、重复性好

可用于花锚药材中齐墩果酸和熊果酸的质量控制。

Abstract

Objective:To establish a determination method of the content of oleanolic acid and ursolic acid in Halenia elliptica by HPLC.Method: The mobie phase was a mixture of methanol-0.5%H3PO4（85∶15）

the column temperature was set 20 ℃

the flow rate was 0.8 mL·min-1

the deterion wave leght was 210 nm.Result: The linear range of oleanolic acid was 0.01-0.05 g·L-1

the linear range of ursolic acid was 0.02-0.1 g·L-1

and the average recovery was 101.17% with RSD 1.90% and 101.79% with RSD 1.65%.Conclusion: The method was rapid

convenient

accurate

and suitable for quality control of oleanolic acid and ursolic in H.eliptica.

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Related Institution

Jiangxi College of Traditional Chinese Medicine

Dept.of Pharmacy,The First Affiliated Hospital of Xinjiang Medical University

Anhui College of Traditional Chinese Medicine,Anhui Key Laboratory of Modernized Chinese Medicine

Nanjing University of Chinese Medicine

Beijing University of Chinese Medicine,School of Chinese Materia Medica

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