The Effect of Buyang Huanwu Decoction on α-SMA in Renal Tubulointerstitial Fibrosis Rats

ZHANG Xuan-ming1; YANG Bai-jing1; YE Dan2; ZHANG Chen-long1; YE Xue-feng3

doi:10.13422/j.cnki.syfjx.2011.23.061

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The Effect of Buyang Huanwu Decoction on α-SMA in Renal Tubulointerstitial Fibrosis Rats

更新时间：2024-09-23

- The Effect of Buyang Huanwu Decoction on α-SMA in Renal Tubulointerstitial Fibrosis Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 23, Pages: 136-140(2011)
- 作者机构：
  
  1. 石河子大学医学院第一附属医院中医一科
  2. 石河子大学医学院第一附属医院老干一科
  3. 成都中医药大学附属医院肾病内科
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.23.061
  CLC： R285.5
- Published：2011
- 稿件说明：
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[1]张选明,杨百京,叶丹,张辰龙,叶学锋.补阳还五汤对肾间质纤维化大鼠α-SMA的作用[J].中国实验方剂学杂志,2011,17(23):136-140.

ZHANG Xuan-ming1, YANG Bai-jing1, YE Dan2, et al. The Effect of Buyang Huanwu Decoction on α-SMA in Renal Tubulointerstitial Fibrosis Rats[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(23): 136-140.
[1]张选明,杨百京,叶丹,张辰龙,叶学锋.补阳还五汤对肾间质纤维化大鼠α-SMA的作用[J].中国实验方剂学杂志,2011,17(23):136-140. DOI： 10.13422/j.cnki.syfjx.2011.23.061.

ZHANG Xuan-ming1, YANG Bai-jing1, YE Dan2, et al. The Effect of Buyang Huanwu Decoction on α-SMA in Renal Tubulointerstitial Fibrosis Rats[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(23): 136-140. DOI： 10.13422/j.cnki.syfjx.2011.23.061.

摘要

目的:观察补阳还五汤对单侧输尿管结扎肾间质纤维化大鼠模型α-平滑肌肌动蛋白（α-SMA）的作用。方法:将SD大鼠随机分为单侧输尿管结扎造模组（UUO）和假手术对照组。造模大鼠再随机分为模型组、补阳还五汤治疗组（中药组）和依那普利组。大鼠分别在第7

28天进行肾功能、HE染色、Masson染色和免疫组化检测。结果:与假手术组相比

模型组大鼠肾功能显著下降;依那普利和中药干预后

肾功能显著提高。光镜显示模型组大鼠出现显著间质纤维化

依那普利和中药干预显著减轻了肾小管损害。各个时间点

模型组肾间质损伤指数和α-SMA的表达与假手术组相比

显著升高。通过依那普利和中药干预在第7天时

显著降低肾间质损伤指数（模型组3.88±0.15

依那普利组2.48±0.43

中药组2.54±0.37

P<0.05）和α-SMA的表达（模型组7.27±1.62

依那普利组5.27±2.18

中药组5.05±1.45

P<0.05）;用药第14

28天后

可进一步降低肾间质损伤指数（14 d

模型组7.65±0.87

依那普利组4.17±0.39

中药组4.24±0.42

P<0.01;28 d

模型组10.86±1.49

依那普利组6.19±0.58

中药组6.94±0.77

P<0.01）和α-SMA的表达（14 d

模型组17.48±4.16

依那普利组10.15±2.85

中药组10.58±3.19

P<0.01;28 d

模型组29.68±7.24

依那普利组17.81±0.58

中药组18.04±4.72

P<0.01）。结论:补阳还五汤可改善单侧输尿管结扎大鼠肾功能和肾小管间质纤维化

降低肾间质损伤指数和α-SMA的表达

对拮抗肾间质纤维化有一定的作用。

Abstract

Objective:To assess the formular Buyang Huanwu decoction on α-smooth muscle actin（α-SMA） in renal tubulointerstitial fibrosis rats caused by unilateral ureteral obstruction.Method: The renal tubulointerstitial fibrosis model was caused by unilateral ureteral obstruction（UUO） in SD rats.The rats were divided into four groups

namely

sham operation group

modeling group

enalapril treated group and Buyang Huanwu decoction treated group.Rats were sacrificed at 7

28 d during experiment period for examination of renal function using light microscopy

hematoxylin-eosin staining

Masson staining for the evaluation of interstitium injury index and immunohistochemistry for α-SMA.Result: The renal function of modeling rats declined compared with sham-group rats.The renal function of rats received either enalapril or Buyang Huanwu decoction significantly improved compared modeling rats.Light microscopy showed that the rats undertook UUO had significant tubulointerstitial fibrosis and both enalapril and sig Buyang Huanwu decoction nificantly improved the renal lesions.At each time point

the interstitium injury index and expression of α-SMA in modeling rats were significantly increased compared with rats received sham operation.Enalapril and Buyang Huanwu decoction significantly decreased the interstitium injury index and expression of α-SMA at d 7（P<0.05）;the index and expression of α-SMA was further decreased in the next three weeks with extreme significance（P<0.01）.Conclusion: The formular Buyang Huanwu decoction can improve renal function and morphology destruction

decrease the interstitium injury index and expression of α-SMA in renal fibrosis rats caused by UUO.The results indicate the formular Buyang Huanwu decoction may have beneficial effects on tubulointerstitial fibrosis.

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ZHANG Xuan-ming

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YE Xue-feng

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Li-qun HE

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Related Institution

Shanghai University of Traditional Chinese Medicine (TCM)

Shuguang Hospital Affiliated to Shanghai University of TCM

Department of Physiology,Henan University of Traditional Chinese Medicine

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