Effects of Weichangan Decoction on TGF-β<sub>1</sub> Induced Epithelial Mesenchymal Transition in Gastric Carcinoma SGC-7901 Cells

SHEN Ke-ping; WANG Hai-yong; HU Bing; LIU Wei

doi:10.13422/j.cnki.syfjx.2011.23.080

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Effects of Weichangan Decoction on TGF-β₁ Induced Epithelial Mesenchymal Transition in Gastric Carcinoma SGC-7901 Cells

更新时间：2024-09-23

- Effects of Weichangan Decoction on TGF-β₁ Induced Epithelial Mesenchymal Transition in Gastric Carcinoma SGC-7901 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 23, Pages: 132-135(2011)
- 作者机构：
  
  上海中医药大学附属龙华医院肿瘤五科
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.23.080
  CLC： R735.2
- Published：2011
- 稿件说明：
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[1]沈克平,王海永,胡兵,刘威.胃肠安对人胃癌SGC-7901细胞TGF-β_1诱导上皮间质转化作用[J].中国实验方剂学杂志,2011,17(23):132-135.

SHEN Ke-ping, WANG Hai-yong, HU Bing, et al. Effects of Weichangan Decoction on TGF-β₁ Induced Epithelial Mesenchymal Transition in Gastric Carcinoma SGC-7901 Cells[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(23): 132-135.
[1]沈克平,王海永,胡兵,刘威.胃肠安对人胃癌SGC-7901细胞TGF-β_1诱导上皮间质转化作用[J].中国实验方剂学杂志,2011,17(23):132-135. DOI： 10.13422/j.cnki.syfjx.2011.23.080.

SHEN Ke-ping, WANG Hai-yong, HU Bing, et al. Effects of Weichangan Decoction on TGF-β₁ Induced Epithelial Mesenchymal Transition in Gastric Carcinoma SGC-7901 Cells[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(23): 132-135. DOI： 10.13422/j.cnki.syfjx.2011.23.080.

摘要

目的:观察胃肠安对人胃癌SGC-7901细胞上皮间质转化的影响。方法:接种SGC-7901细胞贴壁后

按5μg.L-1加入转化生长因子β1（transforming growth factor-β1

TGF-β1）建立人胃癌SGC-7901细胞上皮间质转化模型。同时

胃肠安组按1 000 mg.L-1加入胃肠安药液

继培5 d后

显微镜观察细胞形态

Western blot检测上皮、间质标记基因E-cad

N-cad

vimentin蛋白与相关激酶蛋白激酶B（Protein kinase B

PKB或AKT）、转录因子Twist

Snail蛋白表达情况。结果:较空白组

加入TGF-β1后

SGC-7901细胞发生上皮间质转化

细胞形态拉长变窄且细胞间连接更疏松

上皮标记基因E-cad蛋白相对表达量降低0.241;间质标记基因N-cad

vimentin蛋白相对表达量分别升高0.225

0.371。较模型组

胃肠安作用后

SGC-7901细胞大部仍成卵石状不典型上皮细胞形态

仅少数细胞出现间质细胞形态

上皮标记基因E-cad蛋白相对表达量升高0.210;间质标记基因N-cad

vimentin蛋白相对表达量分别降低0.119

0.124;相关通路AKT表达及磷酸化

转录因子Twist

Snail蛋白相对表达量分别降低0.248

0.085

0.402

0.382。结论:胃肠安可抑制人胃癌SGC-7901细胞上皮间质转化

可能与抑制AKT表达及磷酸化

下调转录因子Twist

Snail蛋白相关。

Abstract

Objective:To investigate the effects of Weichangan decoction

a Chinese herbal formula

on epithelial mesenchymal transition in gastric carcinoma SGC-7901 cells.Method: SGC-7901 cells were seeded and allowed to attach

then the model of epithelial mesenchymal transition was established by transforming growth factor-β1（TGF-β1）（5 μg·L-1） treatment.In Weichangan group

cells were further treated with Weichangan decoction（1 000 mg·L-1）.Five days later

cell morphology was observed by microscope

expressions of E-cad

N-cad

vimentin

Protein kinase B（P-AKT

AKT）

Twist and Snail were detected by western blot.Result: After TGF-β1 treatment

SGC-7901 cells exhibited a narrow elongated and more loosely connected morphology

the relative expression of E-cad was down-regulated 0.241

and the relative expression of N-cad and vimentin were up-regulated 0.225 and 0.371 respectively

suggested epithelial mesenchymal transition was induced in SGC-7901 cells.Compared with model group

SGC-7901 cells in Weichangan group exhibited a pebble-like atypical epithelial morphology.In addition

upon Weichangan decoction treatment

the relative expression of E-cad were up-regulated 0.210

and the relative expression of N-cad

vimentin

P-AKT

AKT

Twist and Snail were down-regulated 0.119

0.124

0.248

0.085

0.402

0.382 respectively.Conclusion:Weichangan decoction has inhibitory effects on epithelial mesenchymal transition in gastric carcinoma SGC-7901 cells

and may related to inhibition of AKT expression and phosphorylation

and down-regulation of Twist and Snail.

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Related Author

SHEN Ke-ping

WANG Hai-yong

HU Bing

LIU Wei

Lilan WANG

Zhongchi XU

Yun LING

Hui FENG

Related Institution

Chinese Medicine Fever Laboratory， School of Chinese Medicine and School of Integrated Chinese and Western Medicine， Nanjing University of Chinese Medicine

The First School of Clinical Medicine of Nanjing University of Chinese Medicine

Second Military Medical University of the Chinese People's Liberation Army

Nanjing BenQ Medical Center

Graduate School， Hebei University of Chinese Medicine

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Effects of Weichangan Decoction on TGF-β1 Induced Epithelial Mesenchymal Transition in Gastric Carcinoma SGC-7901 Cells

DOI：10.13422/j.cnki.syfjx.2011.23.080

摘要

Abstract

关键词

Keywords

references

Effects of Weichangan Decoction on TGF-β₁ Induced Epithelial Mesenchymal Transition in Gastric Carcinoma SGC-7901 Cells