Optimization on Preparation of Epimediumon Total Flavonoids-phospholipid Complex Using Plackett-Burman Design,Box-Behnken Design and Response Surface Methodology

RAN Jian-ming; ZHANG Fa-sen

doi:10.13422/j.cnki.syfjx.2012.04.006

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Optimization on Preparation of Epimediumon Total Flavonoids-phospholipid Complex Using Plackett-Burman Design,Box-Behnken Design and Response Surface Methodology

更新时间：2024-09-23

- Optimization on Preparation of Epimediumon Total Flavonoids-phospholipid Complex Using Plackett-Burman Design,Box-Behnken Design and Response Surface Methodology
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 4, Pages: 69-73(2012)
- 作者机构：
  
  四川省雅安市雅安三九药业有限公司
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.04.006
  CLC： TQ461
- Published：2012
- 稿件说明：
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[1]冉建明,张发森.响应面法优化淫羊藿黄酮磷脂复合物制备工艺[J].中国实验方剂学杂志,2012,18(04):69-73.

RAN Jian-ming, ZHANG Fa-sen. Optimization on Preparation of Epimediumon Total Flavonoids-phospholipid Complex Using Plackett-Burman Design,Box-Behnken Design and Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 69-73.
[1]冉建明,张发森.响应面法优化淫羊藿黄酮磷脂复合物制备工艺[J].中国实验方剂学杂志,2012,18(04):69-73. DOI： 10.13422/j.cnki.syfjx.2012.04.006.

RAN Jian-ming, ZHANG Fa-sen. Optimization on Preparation of Epimediumon Total Flavonoids-phospholipid Complex Using Plackett-Burman Design,Box-Behnken Design and Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 69-73. DOI： 10.13422/j.cnki.syfjx.2012.04.006.

摘要

目的:确定淫羊藿黄酮磷脂复合物制备最佳工艺。方法:采用溶剂挥发法制备淫羊藿黄酮磷脂复合物

以药物与磷脂的结合率为评估标准

采用Plackett-Burman设计对淫羊藿黄酮磷脂复合物制备工艺影响因素的重要性进行考察

用Box-Behnken对重要影响因素的水平进行优化

并且进行多元线性回归与二项式方程拟合

用效应面法预测最佳工艺。结果:投料比例、反应时间与反应温度对磷脂复合物形成影响具有显著性意义;二项式方程拟合度高

预测性好

复相关系数r=0.988

效应面法优选出的最佳工艺为磷脂与淫羊藿总黄酮提取物以3.6∶1投料

反应温度38℃

反应时间2.4 h

主药浓度10g.L-1

最佳工艺试验验证结果与二项式拟合方程预测结果相差<2%。结论:应用响应面法优化得到的淫羊藿黄酮磷脂复合物最佳制备工艺稳定可行

可用于工业生产。

Abstract

Objective:To choose the optimization conditions for the formation of total flavonoids in herba epimediumon complex with phospholipid.Method:Solvent evaporation process was used to prepare total flavonoids in herba epimediumon phospholipid complex.The evaluation criterion is complex ratio.The experiment inspect the significance of influential factor of preparation technology by Plackett-Burman design.Optimized significance of factor and level with the Box-Behnken design.The binomial and linear equations were fitted to the data of overall desirabilities

and the resulting equation was used to produce 3-D response surface graphs

through which optimal formulation were predicted.Result:Total flavonoids and phospholipid ratio

flavonoids concentration and reaction temperature had significance influence to the craft.It showed that the correlation coefficient of second-order quadratic model was high.Correlation coeficient reached 0.988.The optimum conditions for the preparation of total flavonoids in epimediumon complex were as follows:phospholipids-total flavonoids being 3.6∶1

time 2.4 h

temperature at 40 ℃ and concentration of principal agent being 10 g·L-1.The deviation between result of the best craft verification test and the binomial fitting equation forecast value is less than 2.0%.Conclusion:Response surface methodology was successfully used to optimize the preparation of total flavonoids in herba epimediumon phospholipid complex.Optimized process was reliable

stable and available for industrial production.

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Related Institution

Guizhou Provincial Engineering Center for Efficient Utilization of Natural Pharmaceutical Resources， Featured Key Laboratory of Pharmacology and Pharmacological Evaluation of Natural Medicine in Guizhou Colleges and Universities，Guizhou Medical University-Guiyang Municipal Joint Key Laboratory，Key Laboratory of Optimal Utilization of Natural Medicinal Resources，School of Pharmaceutical Sciences，Guizhou Medical University

People's Hospital of Yunyan District

Center for Drug Inspection of Guizhou Medical Products Administration

Guizhou Provincial Food and Drug Inspection Institute

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