ZHANG Xiao-li1, WU Pin-chang1, LIU Yu3, et al. Preparation Technology of Curcumol Liposome by Ethanol Injection Method[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 10-12.
ZHANG Xiao-li1, WU Pin-chang1, LIU Yu3, et al. Preparation Technology of Curcumol Liposome by Ethanol Injection Method[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 10-12. DOI: 10.13422/j.cnki.syfjx.2012.04.018.
Preparation Technology of Curcumol Liposome by Ethanol Injection Method
Objective:To study on optimum formulation and preparation technology of curcumol liposome by ethanol injection method.Method:curcumol liposome were prepared by ethanol injection method;taking entrapment efficiency and stability parameter as indexes
formulation and preparation technology were optimized by orthogonal test;entrapment efficiency of liposome was determined by HPLC.Result:Optimum formulation was:phospholipids 70 g·L-1
phospholipids-cholesterol 8∶1
pH of phosphate buffer 6.0
concentration of phosphate 0.10 mol·L-1;Optimum extraction technology was:probe ultrasonic time 4 min
magnetic stirring speed 30 r·min-1
water bath temperature 50 ℃
appearance of prepared curcumol liposome was uniform round
with entrapment efficiency was 62.91%
average particle size was 124 nm and average zeta-potential was-26.7 mV.Conclusion:preparation process of ethanol injection was simple and easy to control.optimized formulation and preparation of curcumol lipsome was stable and feasible.
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ZHANG Xiao-li
WU Pin-chang
LIU Yu
LI Tie-fu
ZHANG Qiuyan
LI Ying
GAO Yunxiao
HU Longxiao
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