LIU Yan-shan, XIN Chang-ying, XIONG Zhi-li, et al. Optimization of Extraction Technology of Gushudan by Smashing Extraction Method[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 20-24.
LIU Yan-shan, XIN Chang-ying, XIONG Zhi-li, et al. Optimization of Extraction Technology of Gushudan by Smashing Extraction Method[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 20-24. DOI: 10.13422/j.cnki.syfjx.2012.04.021.
Optimization of Extraction Technology of Gushudan by Smashing Extraction Method
摘要
目的:优化骨疏丹闪式提取工艺。方法:以骨疏丹中总黄酮、总香豆素含量
柚皮苷、朝藿定B、淫羊藿苷、蛇床子素和丹参酮ⅡA含量之和及浸膏率为评价指标
对骨疏丹的闪式提取工艺进行考察。结果:最佳提取工艺为12倍量75%乙醇
闪式提取3次
每次2 min。按最佳工艺所得总黄酮质量分数26.27 mg.g-1
总香豆素17.93 mg.g-1
柚皮苷、朝藿定B等5个成分含量之和9.213 mg.g-1
浸膏率23.50%。结论:优选出的闪式提取方法稳定性好、简便可行。
Abstract
Objective:To optimize extraction technology of Gushudan by smashing extraction method.Method:Smashing extraction technology of Gushudan was studied by the content of total flavonoids and total coumarins
the total contents of naringin
epimedin B
icariin
osthole and tanshinoneⅡA
and extract ratio from Gushudan as indexes.Result:Optimum extraction technology was as follows:smashing extracted 3 times with 12 times the amount of 75% ethanol as dissolvent
2 minutes every time.The content of total flavonoids
total coumarins
total contents of naringin
epimedin B
icariin
osthole and tanshinoneⅡA
and extract ratio were 26.27
17.93
9.213 mg·g-1
and 23.50% respectively.Conclusion:Optimum smashing extraction technology was stable and easy.