WU Si, QIU Ting, CHEN Peng, et al. Methods Comparative of Removal of Protein for Polysaccharide from Liuwei Dihuang Biological Preparation[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 56-59.
WU Si, QIU Ting, CHEN Peng, et al. Methods Comparative of Removal of Protein for Polysaccharide from Liuwei Dihuang Biological Preparation[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 56-59. DOI: 10.13422/j.cnki.syfjx.2012.04.026.
Methods Comparative of Removal of Protein for Polysaccharide from Liuwei Dihuang Biological Preparation
Objective:To comparative study on removal method of protein for polysaccharide from Liuwei Dihuang biological preparation
and investigate effect on yield and purity of polysaccharide by different methods.Method:Sevage method
TCA-butanol precipitation method and tannin acid precipitation method were taken to deproteinized of Liuwei Dihuang biological preparation
and compared effect of three deproteinization methods by deproteinization rate and polysaccharides retention rate.Result:Deproteinizationhe effect of TCA-butanol precipitation method was the best
optimum conditions were:volume ratio of TCA-butanol reagent was 1∶2
ratio of TCA-butanol reagents was 1∶5
vibration time was 30 min
standing for 1.0 h
which showed highest efficient of deproteinization for polysaccharides and higher polysaccharides retention rate.Conclusion:TCA-butanol method was optimum technology of removing protein from Liuwei Dihuang biological preparation.
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Related Author
WU Si
QIU Ting
CHEN Peng
LI Tian-he
TANG Pei-lian
ZHAO Yue
LI Qiang
DING Ai-ling
Related Institution
Guangdong College of Phamarcy
He’nan University of Traditional Chinese Medicine
Jiangxi Jingde Chinese Traditional Medicine Co. Ltd.
Jiangxi University of Chinese Medicine
School of Pharmacy, Chengdu University of Traditional Chinese Medicine,State Key Laboratory of Southwestern Chinese Medicine Resources, Key Laboratory of Standardization of Chinese Herbal Medicine,Ministry of Education