Extraction and Purification Technology of Total Flavonoids for Scutellaria baicalensis and Sedum sarmentosum from Longchaifang

PEI Dan1; ZHAO Ming1; OUYANG Zhen1; CAO Xu1; YANG Xi-hui1; LIU Yue-qin1

doi:10.13422/j.cnki.syfjx.2012.04.027

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Extraction and Purification Technology of Total Flavonoids for Scutellaria baicalensis and Sedum sarmentosum from Longchaifang

更新时间：2024-09-23

- Extraction and Purification Technology of Total Flavonoids for Scutellaria baicalensis and Sedum sarmentosum from Longchaifang
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 4, Pages: 59-63(2012)
- 作者机构：
  
  1. 江苏大学药学院
  2. 江苏大学附属医院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.04.027
  CLC： R284
- Published：2012
- 稿件说明：
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[1]裴丹,赵明,欧阳臻,曹旭,杨锡辉,刘月琴.龙柴方中黄芩、垂盆草总黄酮的提取纯化工艺[J].中国实验方剂学杂志,2012,18(04):59-63.

PEI Dan1, ZHAO Ming1, OUYANG Zhen1, et al. Extraction and Purification Technology of Total Flavonoids for Scutellaria baicalensis and Sedum sarmentosum from Longchaifang[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 59-63.
[1]裴丹,赵明,欧阳臻,曹旭,杨锡辉,刘月琴.龙柴方中黄芩、垂盆草总黄酮的提取纯化工艺[J].中国实验方剂学杂志,2012,18(04):59-63. DOI： 10.13422/j.cnki.syfjx.2012.04.027.

PEI Dan1, ZHAO Ming1, OUYANG Zhen1, et al. Extraction and Purification Technology of Total Flavonoids for Scutellaria baicalensis and Sedum sarmentosum from Longchaifang[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 59-63. DOI： 10.13422/j.cnki.syfjx.2012.04.027.

摘要

目的:优选龙柴方中黄芩、垂盆草总黄酮的提取纯化工艺。方法:以总黄酮含量为指标

采用正交试验法优选总黄酮提取纯化工艺条件。大孔树脂纯化总黄酮

以静态吸附及解析试验筛选树脂型号;通过动态吸附相关指标考察最佳洗脱条件。结果:最佳提取工艺为15倍量60%乙醇

85℃水浴回流提取2次

每次2 h。最佳纯化工艺条件为采用AB-8型大孔树脂

上样浓度为0.11 g.mL-1

吸附速度3 BV.h-1

5 BV水洗除杂

8 BV 70%乙醇洗脱。结论:优选工艺得到的总黄酮含量较高

工艺简单

适宜工业化生产。

Abstract

Objective:To optimize extraction and purification technology of total flavonoids for S.baicalensis and S.sarmentosum from Longchaifang.Method:The content of total flavonoids were selected as index

extraction and purification technology condition of total flavonoids was optimized by orthogonal test.Total flavonoids was purified by macroporous resin

screened optimum purification macroporous resin by static adsorption and desorption test;Optimum elution condition was examined by related indicators of dynamic adsorption.Result:Optimum extraction process was as follows:reflux extracted 2 times with 15 times the anount of 60% ethanol at 85 ℃

2 h each time.Optimum purification technology was as follows:AB-8 macroporous resin was adopted

concentration of sample was 0.11 g·mL-1

adsorptive speed was 3 BV·h-1

adsorbing column was eluted with 5 BV waterand 8 BV 70% ethanol.Conclusion:The content of the total flavonoids was high by optimization technology.This technique was simple and suitable for modern production.

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Related Author

PEI Dan

ZHAO Ming

OUYANG Zhen

CAO Xu

YANG Xi-hui

LIU Yue-qin

ZHU Xin-ting

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Related Institution

Guilin Medical University

Affiliated Hospital of Guilin Medical College

Zhejiang Chinese Medical University

Zhejiang Province Hospital of Traditionl Chinese Medicine

Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences and Peking Union Medical College

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