Determination of Baicalein-7-O-gentiobioside and Baicalin in Senmen Oroxyli from Guangxi Province by HPLC

CAO Yang-yang1; YAN Ren-yi2; YANG-Bin2; HUANG Lu-qi2

doi:10.13422/j.cnki.syfjx.2012.04.030

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Determination of Baicalein-7-O-gentiobioside and Baicalin in Senmen Oroxyli from Guangxi Province by HPLC

更新时间：2024-09-23

- Determination of Baicalein-7-O-gentiobioside and Baicalin in Senmen Oroxyli from Guangxi Province by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 4, Pages: 82-84(2012)
- 作者机构：
  
  1. 首都医科大学中医药学院
  2. 中国中医科学院中药研究所
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.04.030
  CLC： R284.1
- Published：2012
- 稿件说明：
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[1]曹阳阳,晏仁义,杨滨,黄璐琦.HPLC测定广西木蝴蝶中木蝴蝶苷B和黄芩苷的含量[J].中国实验方剂学杂志,2012,18(04):82-84.

CAO Yang-yang1, YAN Ren-yi2, YANG-Bin2, et al. Determination of Baicalein-7-O-gentiobioside and Baicalin in Senmen Oroxyli from Guangxi Province by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 82-84.
[1]曹阳阳,晏仁义,杨滨,黄璐琦.HPLC测定广西木蝴蝶中木蝴蝶苷B和黄芩苷的含量[J].中国实验方剂学杂志,2012,18(04):82-84. DOI： 10.13422/j.cnki.syfjx.2012.04.030.

CAO Yang-yang1, YAN Ren-yi2, YANG-Bin2, et al. Determination of Baicalein-7-O-gentiobioside and Baicalin in Senmen Oroxyli from Guangxi Province by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 82-84. DOI： 10.13422/j.cnki.syfjx.2012.04.030.

摘要

目的:建立木蝴蝶药材中木蝴蝶苷B和黄芩苷同时定量的HPLC法。方法:采用Agilent Zorbax Extend-C18色谱柱（4.6 mm×250 mm

5μm）

流动相为乙腈-甲醇-0.3%磷酸溶液梯度洗脱

流速1 mL.min-1

柱温30℃

检测波长280 nm。结果:木蝴蝶苷B和黄芩苷的质量在0.061～1.525μg（r=0.999 9）和0.276～6.900μg（r=1.000 0）与峰面积呈良好的线性关系

平均回收率（n=6）分别为98.06%

100.35%。结论:所建立的HPLC法可用于木蝴蝶药材中木蝴蝶苷B和黄芩苷的含量测定

为木蝴蝶药材的质量控制提供参考依据。

Abstract

Objective:To establish an HPLC method of determining baicalein-7-O-gentiobioside and baicalin in Senmen oroxyli simultaneously.Method:The separation was performed at 30 ℃ on a Zorbax Extend-C18 column（4.6 mm × 250 mm

5 μm） with acetonitrile-methanol-0.3% H3PO4as the mobile phase.The flow ratewas 1 mL.min-1.The detection wavelength was at 280 nm.Result:The linear rage of baicalein-7-O-gentiobioside and baicalin was 0.061-1.525 μg（r=0.999 9） and 0.276-6.900 μg（r=1.000 0）

respectively.The average recoveries（n=6） of baicalein-7-O-gentiobioside and baicalin were 98.06% and 100.35%

respectively.Conclution:The established method can be used for quantitative analysis of baicalein-7-O-gentiobioside and baicalin in Senmen oroxyli

which can provide a reference for quality control of this traditional Chinese medicine.

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Related Institution

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