Detemination of Trigonelline in Pinellia ternate by UPLC

CHENG Zai-xing1; YAN Tong-meng3; CHEN Hong1; LUO Hui3; LIU Zhi-qiang4; LIN Na2; LIU Zhong-qiu3

doi:10.13422/j.cnki.syfjx.2012.04.031

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Detemination of Trigonelline in Pinellia ternate by UPLC

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- Detemination of Trigonelline in Pinellia ternate by UPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 4, Pages: 85-87(2012)
- 作者机构：
  
  1. 福建中医药大学药学院
  2. 中国中医科学院中药研究所
  3. 南方医科大学药学院
  4. 中国科学院长春应用化学研究所
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.04.031
  CLC： R284.1
- Published：2012
- 稿件说明：
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[1]程再兴,严通萌,陈红,罗辉,刘志强,林娜,刘中秋.UPLC测定半夏中胡芦巴碱的含量[J].中国实验方剂学杂志,2012,18(04):85-87.

CHENG Zai-xing1, YAN Tong-meng3, CHEN Hong1, et al. Detemination of Trigonelline in Pinellia ternate by UPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 85-87.
[1]程再兴,严通萌,陈红,罗辉,刘志强,林娜,刘中秋.UPLC测定半夏中胡芦巴碱的含量[J].中国实验方剂学杂志,2012,18(04):85-87. DOI： 10.13422/j.cnki.syfjx.2012.04.031.

CHENG Zai-xing1, YAN Tong-meng3, CHEN Hong1, et al. Detemination of Trigonelline in Pinellia ternate by UPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 85-87. DOI： 10.13422/j.cnki.syfjx.2012.04.031.

摘要

目的:采用超高效液相（UPLC）及亲水作用色谱法（HILIC

hydrophilic interaction chromatography）测定半夏中胡芦巴碱的含量

建立半夏药材质量评价方法。方法:半夏细粉经50%的甲醇超声提取后

采用ACQUITY UPLC BEH HILIC色谱柱（2.1 mm×100 mm

1.7μm）进行胡芦巴碱含量的测定

流动相为乙腈-10 mmol.L-1醋酸铵（80∶20）

流速0.4 mL.min-1

检测波长265 nm

进样量10μL。结果:盐酸胡芦巴碱在1.125～72 nmol.mL-1线性关系良好（r=0.999 6

n=7）

平均回收率为99.7%（RSD 1.4%）。结论:该方法专属性强、重复性好、稳定、可控

可作为半夏药材质量控制的方法。

Abstract

Objective:To develop UPLC and a hydrophilic interaction chromatography（HILIC） method for the determination of trigonelline in Pinellia ternate.Method:After extracting trigonelline in the fine powder of Pinellia ternate by ultrasonic extraction with 50% methanol

the separation was performed at ACQUITY UPLC BEH HILIC column（2.1 mm × 100 mm

1.7 μm）.The mobile phase consisted of acetonitrile-10 mmol.L-1 ammonium acetate（80∶ 20）

and the flow rate was 0.4 mL.min-1.The detection wave length was set at 265 nm.The sampling volume was 10 μL.Result:The method has good linearity in the range of 1.125-72 nmol.mL-1 for trigonelline （r=0.999 6

n=7）.The average recovery was 99.7%（RSD 1.4%）.Conclusion:The method is specific

accurate and stable.It can be used for determination of trigonelline in Pinellia ternate.

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