Determination of Lethariella cladonioides Polysaccharide from Tibet

ZHUANG Xiao-wei1; LIU Xiu-fang1; MAO Gui-yuan1; CHEN Ben-chao1; CAI Guang-ming2; XIA Xin-hua1

doi:10.13422/j.cnki.syfjx.2012.04.036

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Determination of Lethariella cladonioides Polysaccharide from Tibet

更新时间：2024-09-23

- Determination of Lethariella cladonioides Polysaccharide from Tibet
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 4, Pages: 103-106(2012)
- 作者机构：
  
  1. 解放军302医院中药研究所
  2. 湖南中医药大学药学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.04.036
  CLC： R284.1
- Published：2012
- 稿件说明：
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[1]庄筱葳,刘秀芳,毛贵元,陈本超,蔡光明,夏新华.西藏红雪茶多糖含量测定[J].中国实验方剂学杂志,2012,18(04):103-106.

ZHUANG Xiao-wei1, LIU Xiu-fang1, MAO Gui-yuan1, et al. Determination of Lethariella cladonioides Polysaccharide from Tibet[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 103-106.
[1]庄筱葳,刘秀芳,毛贵元,陈本超,蔡光明,夏新华.西藏红雪茶多糖含量测定[J].中国实验方剂学杂志,2012,18(04):103-106. DOI： 10.13422/j.cnki.syfjx.2012.04.036.

ZHUANG Xiao-wei1, LIU Xiu-fang1, MAO Gui-yuan1, et al. Determination of Lethariella cladonioides Polysaccharide from Tibet[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 103-106. DOI： 10.13422/j.cnki.syfjx.2012.04.036.

摘要

目的:研究西藏红雪茶多糖含量测定的方法。方法:采用苯酚-硫酸法测定总糖含量

5-二硝基水杨酸（DNS）法测定还原糖含量

两者的差值即为多糖含量。结果:总糖标准曲线方程为OD=0.051 4C-0.0146（r=0.999 2）

还原糖标准曲线方程为OD=0.022 9C-0.086（r=0.999 9）

两者分别在2.5～12.5

8～40 mg.L-1有良好的线性关系。测得LC

LCA

LCP多糖含量分别为63.6%

82.6%

57.6%。结论:该方法操作快速、简便、灵敏、准确、重复性好。

Abstract

Objective:To establish a method for the determination of polysaccharide in Lethariella cladonioides.Method:Using microplate reader

the content of total sugar was determined by means of phenol-sulfuric acid method and 3

5-dinitro salicylic acid（DNS） method was used for the determination of reducing sugar

the difference between the two was the polysaccharide content.Results:The total sugar standard curve equation was OD=0.051 4 C-0.014 6（r=0.999 2） and reducing sugar standard curve equation was OD =0.022 9C-0.086（r=0.999 9）.Respectively the linear range was 2.5-12.5 mg.L-1 and 8-40 mg.L-1.Thepolysaccharide content of LC

LCA

LCP were 63.6%

82.6%

57.6%.Conclusion:The method is fast

simple

sensitive

accurate and reproducible.

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