Determination of Chlorogenic Acid and Baicalin in Kunlun Chrysanthemum by HPLC

ZHANG Yan-li; WANG Yan; LI Xin-xia; ZHI Ling; Aytulun.Simayil

doi:10.13422/j.cnki.syfjx.2012.04.037

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Determination of Chlorogenic Acid and Baicalin in Kunlun Chrysanthemum by HPLC

更新时间：2024-09-23

- Determination of Chlorogenic Acid and Baicalin in Kunlun Chrysanthemum by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 4, Pages: 107-109(2012)
- 作者机构：
  
  新疆医科大学药学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.04.037
  CLC： R286
- Published：2012
- 稿件说明：
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[1]张彦丽,王艳,李新霞,支玲,阿依吐伦·斯马义.高效液相色谱法测定昆仑雪菊中绿原酸和黄芩苷的含量[J].中国实验方剂学杂志,2012,18(04):107-109.

ZHANG Yan-li, WANG Yan, LI Xin-xia, et al. Determination of Chlorogenic Acid and Baicalin in Kunlun Chrysanthemum by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 107-109.
[1]张彦丽,王艳,李新霞,支玲,阿依吐伦·斯马义.高效液相色谱法测定昆仑雪菊中绿原酸和黄芩苷的含量[J].中国实验方剂学杂志,2012,18(04):107-109. DOI： 10.13422/j.cnki.syfjx.2012.04.037.

ZHANG Yan-li, WANG Yan, LI Xin-xia, et al. Determination of Chlorogenic Acid and Baicalin in Kunlun Chrysanthemum by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 107-109. DOI： 10.13422/j.cnki.syfjx.2012.04.037.

摘要

目的:建立新疆昆仑雪菊中绿原酸和黄芩苷的HPLC含量测定方法。方法:采用Inertsil ODS-SP色谱柱（4.6 mm×150 mm

5μm）;流动相甲醇-0.05%磷酸水溶液（三乙胺调pH为3.0）梯度洗脱;流速0.6 mL.min-1

检测波长330nm

柱温25℃。结果:绿原酸线性回归方程为A=55 102C-24 808

r=0.999 8（n=6）

线性范围为0.45～15.00 mg.L-1

平均回收率为102.58%.（RSD 0.93%

n=9）;黄芩苷回归方程为A=25 599C-16 099

r=0.999 7（n=6）

线性范围为0.90～30.00 mg.L-1

平均回收率为104.90%（RSD 1.12%

n=9）。结论:本方法稳定

重复性好

操作简单

是检测新疆昆仑雪菊中绿原酸与黄芩苷的较理想方法。

Abstract

Objective:To establish a HPLC method for the determination chlorogenic acid and baicalin in Kunlun Chrysanthemum.Method:The HPLC method was used.The sample was analyzed on an Inertsil ODS-SP（4.6 mm × 150 mm

5 μm） column at 25 temperature

with mobile phase consisting of methanol and 0.05% phosphoric acid（pH 3.0） by gradient eluteion at the flow rate of 0.6 mL.min-1

The wavelength was at 330 nm.Result:The regression equation of chlorogenic acid was linear in the range of 0.45-15.00 mg.L-1

A =55 102C-24 808

r = 0.999 8（n = 6）.The average recovery was 102.58%（RSD 0.93%）.The regression equation of baicalin was linear in the range of 0.90-30.00 mg.L-1

A = 25 599C-16 099

r = 0.999 7（n = 6）.The average recovery was 104.90%

（RSD 1.12%）.Conclusion:The method was stable and accurate

it is suitable to determine chlorogenic acid and baicalin in Kunlun Chrysanthemum

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