LIU Yu-qiang, ZHEN Bi-xian, CAI Qian. Determination of Atractylenolide Ⅱ in Crude Drugs and Slices of Atractylodes macrocephala Koidz.from Different Areas by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 120-122.
LIU Yu-qiang, ZHEN Bi-xian, CAI Qian. Determination of Atractylenolide Ⅱ in Crude Drugs and Slices of Atractylodes macrocephala Koidz.from Different Areas by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 120-122. DOI: 10.13422/j.cnki.syfjx.2012.04.041.
Determination of Atractylenolide Ⅱ in Crude Drugs and Slices of Atractylodes macrocephala Koidz.from Different Areas by HPLC
摘要
目的:采用HPLC测定不同地区白术药材及饮片中白术内酯Ⅱ的含量。方法:采用Tigerkin C18(4.6 mm×200 mm
Objective:To determine the content of atractylenolide Ⅱ in crude drugs and slices of Atractylodes macrocephala from different areas by HPLC.Method:The samples were separated on Tigerkin C18column(4.6 mm×200 mm
5 μm)with the mobile phase of methanol-water(77∶23).Flow rate was 1.0 mL·min-1.The detection wavelength was set at 276 nm.Column temperature was maintained at 30 ℃.Result:The contents of atractylenolide Ⅱ in crude drugs from 10 places of origin and slices and processing slices of A.macrocephala from 20 regions was determined.Conclusion:The contents of atractylenolide Ⅱ in crude drugs and slices of A.macrocephala from different regions was different.
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Related Author
LIU Yu-qiang
ZHEN Bi-xian
CAI Qian
DUAN Haohan
JIA Hao
LEI Jingwei
YANG Chunjing
XIE Caixia
Related Institution
Henan Engineering Research Center for Quality Control and Evaluation of Traditional Chinese Medicine(TCM),School of Pharmacy,Henan University of Chinese Medicine
Luohe Medical College
Third Affiliated Hospital of Henan University of Chinese Medicine
Tianjin University of Traditional Chinese Medicine
Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences