Effect of Gegen Powder on CYP450 Content of Liver Microsomal and Activity of CYP2E1 in Mice Acute Alcohol-induced Liver Injury

TIAN Wei-yi1; WANG Qing-xue2; WANG Wen-jia1; YANG Zhu2

doi:10.13422/j.cnki.syfjx.2012.04.059

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Effect of Gegen Powder on CYP450 Content of Liver Microsomal and Activity of CYP2E1 in Mice Acute Alcohol-induced Liver Injury

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- Effect of Gegen Powder on CYP450 Content of Liver Microsomal and Activity of CYP2E1 in Mice Acute Alcohol-induced Liver Injury
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 4, Pages: 183-186(2012)
- 作者机构：
  
  1. 贵阳中医学院免疫教研室
  2. 贵阳中医学院中医基础教研室
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.04.059
  CLC： R285.5
- Published：2012
- 稿件说明：
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[1]田维毅,王庆学,王文佳,杨柱.葛根散对急性酒精性肝损伤小鼠肝微粒体CYP450含量及CYP2E1活性的影响[J].中国实验方剂学杂志,2012,18(04):183-186.

TIAN Wei-yi1, WANG Qing-xue2, WANG Wen-jia1, et al. Effect of Gegen Powder on CYP450 Content of Liver Microsomal and Activity of CYP2E1 in Mice Acute Alcohol-induced Liver Injury[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 183-186.
[1]田维毅,王庆学,王文佳,杨柱.葛根散对急性酒精性肝损伤小鼠肝微粒体CYP450含量及CYP2E1活性的影响[J].中国实验方剂学杂志,2012,18(04):183-186. DOI： 10.13422/j.cnki.syfjx.2012.04.059.

TIAN Wei-yi1, WANG Qing-xue2, WANG Wen-jia1, et al. Effect of Gegen Powder on CYP450 Content of Liver Microsomal and Activity of CYP2E1 in Mice Acute Alcohol-induced Liver Injury[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 183-186. DOI： 10.13422/j.cnki.syfjx.2012.04.059.

摘要

目的:考察经典解酒方葛根散对急性酒精性肝损伤小鼠肝微粒体细胞色素P450（CYP450）含量及细胞色素P450-2E1（CYP2E1）活性的影响。方法:实验小鼠分葛根散高、中、低剂量组、正常对照组和模型对照组

各给药组分别给予葛根散水煎剂（按生药量计

5 g.kg-1）ig

对照组代以等体积蒸馏水

每天1次

连续10 d;每天给药30 min后

模型对照组和各给药组以15 mL.kg-1 56%白酒ig

正常对照组代以等体积蒸馏水

以血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）及肝脏系数为参考指标

制备急性酒精性肝损伤小鼠模型;取小鼠肝组织检测葛根散对肝微粒体CYP450含量及CYP2E1活性的影响。结果:与正常对照组比较:模型对照组血清ALT、AST水平及肝脏系数显著升高（P<0.01）

肝微粒体蛋白含量无明显变化

CYP450含量及CYP2E1活性显著提高（P<0.01）。与模型对照组比较:葛根散各剂量组血清ALT

AST水平及大剂量组肝脏系数呈明显降低（P<0.05或P<0.01）

肝微粒体蛋白含量无明显变化

CYP450含量明显增多（P<0.01）且有剂量差异性（P<0.05）

CYP2E1活性显著降低（P<0.01）且有剂量差异性（P<0.01）。结论:葛根散对急性酒精性肝损伤小鼠肝功能具有良性干预作用

能提高酒精性肝损伤小鼠肝微粒体CYP450含量和降低CYP2E1活性。

Abstract

Objective:To observe the effects of Jiejiu recipes of Gegen powder on the cytochrome P450（CYP450） content of liver microsomal and the activity CYP2E1 in mice acute alcohol-induced liver injury model.Method:Mouse were divided into normal control group

model group and Gegen powder high

medium nad low dose groups.Gegen powder of 20

5 g·kg-1

was given once a day and continuing ten days.After 30 minutes of administration everyday

the mouse of model group and treatment groups were orally given 56% alcohol of 15 mL·kg-1 to establish acute alcohol-induced liver injury model

and the level of ALT

AST in serum and liver index were observed;Content of CYP450 and the activity of CYP2E1 were detected.Result:Compared with normal control group

the level of ALT

AST and liver index in model group had obvious enhance（P<0.01）

the content of microsomal protein had no significant change

the content of CYP450 and the activity of CYP2E1 were significantly raised（P<0.01）.Compared with model group

the level of ALT

AST and liver index in Gegen powder groups had obvious reduction（P<0.05 or P<0.01）

the content of microsomal protein had no significant change

the content of CYP450 was increased significantly（P<0.01）

the activity of CYP2E1 was significantly decreased（P<0.01） and there was a significant difference between different dosage groups（P<0.05）.Conclusion:Gegen powder indicates the therapeutic effect on acute alcohol-induced liver injury

it can be contribute to increaseing the content of CYP450 and reduceing the activity of CYP2E1

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