Controlling Effects of Neiyi Fang on Expression of mRNA and Protein of TGF-β<sub>1</sub> in Model Rats with EMS Iesions

FU Jin-rong; ZHENG Jin; WANG Fang; TIAN Li-xia; XU Hua-yun; JIANG Hui

doi:10.13422/j.cnki.syfjx.2012.04.068

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Controlling Effects of Neiyi Fang on Expression of mRNA and Protein of TGF-β₁ in Model Rats with EMS Iesions

更新时间：2024-09-23

- Controlling Effects of Neiyi Fang on Expression of mRNA and Protein of TGF-β₁ in Model Rats with EMS Iesions
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 4, Pages: 227-229(2012)
- 作者机构：
  
  上海中医药大学附属龙华医院妇科
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.04.068
  CLC： R285.5
- Published：2012
- 稿件说明：
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[1]付金荣,郑锦,王芳,田立霞,许华云,姜卉.内异方对模型大鼠内异灶组织TGF-β_1的mRNA及蛋白表达的调控作用[J].中国实验方剂学杂志,2012,18(04):227-229.

FU Jin-rong, ZHENG Jin, WANG Fang, et al. Controlling Effects of Neiyi Fang on Expression of mRNA and Protein of TGF-β₁ in Model Rats with EMS Iesions[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 227-229.
[1]付金荣,郑锦,王芳,田立霞,许华云,姜卉.内异方对模型大鼠内异灶组织TGF-β_1的mRNA及蛋白表达的调控作用[J].中国实验方剂学杂志,2012,18(04):227-229. DOI： 10.13422/j.cnki.syfjx.2012.04.068.

FU Jin-rong, ZHENG Jin, WANG Fang, et al. Controlling Effects of Neiyi Fang on Expression of mRNA and Protein of TGF-β₁ in Model Rats with EMS Iesions[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 227-229. DOI： 10.13422/j.cnki.syfjx.2012.04.068.

摘要

目的:探讨内异方对实验性大鼠子宫内膜异位灶组织转化生长因子-β1（TGF-β1）的mRNA及蛋白表达的调控作用。方法:SD大鼠采用自身种植法建立子宫内膜异位症（EMS）模型

分为假手术组、模型组、内异方低、高剂量（含生药25.2

50.4 g.kg-1.d-1）、孕三烯酮对照组（2.5 mg.kg-1.d-1）

ig 3周后

应用RT-PCR检测内异灶组织中TGF-β1 mRNA的表达

Western印迹检测内异灶组织TGF-β1的蛋白表达。结果:模型组TGF-β1 mRNA及蛋白表达水平明显高于假手术组（P<0.01）

内异方组明显低于模型组（P<0.01）

与孕三烯酮对照组相比无明显差异。结论:大鼠模型"异灶中TGF-β1 mRNA及蛋白的表达升高

内异方通过下调TGF-β1 mRNA及蛋白的表达

起到抗EMS的作用。

Abstract

Objective:To investigate the controlling effects of Neiyi Fang on expression of mRNA and protein of（TGF-β1） in endometriosis（EMS） lesions of model rats.Method:The model of EMS was duplicated in SD rats

rats were divided into sham group

model group

low-dose of Neiyi Fang group（25.2 g·kg-1·d-1）

high-dose of Neiyi Fang group（50.4 g·kg-1·d-1） and western medicine（gestrinone） group（2.5 mg·kg-1·d-1）.RT-PCR was used to detect the expression of mRNA of TGF-β1 in EMS lesions.Western blot was used to detect the expression of protein of TGF-β1.Result:The level of expression of mRNA and protein of TGF-β1 in the model group was clearly higher than that in the sham group（P<0.01）

while in the Neiyi Fang group

the level was clearly lower than that in the model group（P<0.01）.The level in the Neiyi Fang group was no significant difference than in the western medicine group.Conclusion:The expression of mRNA and protein of TGF-β1 in EMS lesions of model rats were increased.Neiyi Fang resisted EMS through decreasing the expression of mRNA and protein of TGF-β1.

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Related Author

JIANG Hui

XU Hua-yun

TIAN Li-xia

WANG Fang

ZHENG Jin

FU Jin-rong

ZHAO Jin-ru

REN Yan-ling

Related Institution

Institute of Chinese Traditional and Western Medicine,Lanzhou University

Hospital of Orthpedic and Traumatic of Traditional Chinese Medicine,Chengguan District of Lanzhou City Gansu Province

Wangjiashan Hospital of Jingyuan Coal Company

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Controlling Effects of Neiyi Fang on Expression of mRNA and Protein of TGF-β1 in Model Rats with EMS Iesions

DOI：10.13422/j.cnki.syfjx.2012.04.068

摘要

Abstract

关键词

Keywords

references

Controlling Effects of Neiyi Fang on Expression of mRNA and Protein of TGF-β₁ in Model Rats with EMS Iesions