Hepato-protective Activity of Glycyrrhizin,Liquiritin and Isoliquiritigenin on HL-7702 Cells Injury Induced by Acetaminophen

CHEN Yun-hua1; WAN Xin2; SUN Jian-ning3; WANG Wen-quan3; ZHANG Shuo-feng3

doi:10.13422/j.cnki.syfjx.2012.04.072

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Hepato-protective Activity of Glycyrrhizin,Liquiritin and Isoliquiritigenin on HL-7702 Cells Injury Induced by Acetaminophen

更新时间：2024-09-23

- Hepato-protective Activity of Glycyrrhizin,Liquiritin and Isoliquiritigenin on HL-7702 Cells Injury Induced by Acetaminophen
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 4, Pages: 245-248(2012)
- 作者机构：
  
  1. 北京城市学院生物医药学部
  2. 国家知识产权局专利审查协作中心化学处
  3. 北京中医药大学中药学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.04.072
  CLC： R285.5
- Published：2012
- 稿件说明：
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[1]陈云华,万新,孙建宁,王文全,张硕峰.甘草酸、甘草苷、异甘草素对醋氨酚人肝细胞损伤模型的保护作用比较[J].中国实验方剂学杂志,2012,18(04):245-248.

CHEN Yun-hua1, WAN Xin2, SUN Jian-ning3, et al. Hepato-protective Activity of Glycyrrhizin,Liquiritin and Isoliquiritigenin on HL-7702 Cells Injury Induced by Acetaminophen[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 245-248.
[1]陈云华,万新,孙建宁,王文全,张硕峰.甘草酸、甘草苷、异甘草素对醋氨酚人肝细胞损伤模型的保护作用比较[J].中国实验方剂学杂志,2012,18(04):245-248. DOI： 10.13422/j.cnki.syfjx.2012.04.072.

CHEN Yun-hua1, WAN Xin2, SUN Jian-ning3, et al. Hepato-protective Activity of Glycyrrhizin,Liquiritin and Isoliquiritigenin on HL-7702 Cells Injury Induced by Acetaminophen[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(4): 245-248. DOI： 10.13422/j.cnki.syfjx.2012.04.072.

摘要

目的:建立并验证醋氨酚人肝细胞损伤模型

进一步研究甘草酸、甘草苷、异甘草素的保肝作用。方法:20 mmol.L-1醋氨酚作用HL-7702正常人肝细胞3 h

以四氮溴盐（MTT）为考察指标

通过阳性药物双环醇验证该细胞模型并获得量效参考值

进而研究不同质量浓度的甘草酸、甘草苷、异甘草素对人肝细胞损伤的保护作用。结果:醋氨酚人肝细胞损伤模型成功、方法可靠

甘草酸、甘草苷、异甘草素3种成分对人肝细胞醋氨酚损伤均具一定的保护作用。甘草酸的最低有效浓度为0.001 6μmol.L-1

保肝的最大效能为47.0%

达到最大效能的浓度为0.2μmol.L-1。甘草苷的最低有效浓度为0.4μmol.L-1

保肝的最大效能为22.9%

达到最大效能的浓度为2μmol.L-1。异甘草素的最低有效浓度为0.4μmol.L-1

保肝的最大效能为72.6%

达到最大效能的浓度为250μmol.L-1。结论:提供一种新的体外肝细胞损伤模型;在甘草用于保肝相关作用时

单纯以甘草酸、甘草苷作为质量评价指标是不够的。

Abstract

Objective:To study the hepato-protective activity of glycyrrhizin

liquiritin and isoliquritigenin on a new HL-7702 cells injury model induced by acetaminophen.Method:HL-7702 cells injury induced by 20 mmol·L-1 acetaminophen for 3 hours were chosen as the liver cells injury model and the effect of glycyrrhizin

liquiritin and isoliquritigenin was assayed.The main index was MTT.Result:The new HL-7702 cells injury model induced by acetaminophen was successful.Glycyrrhizin

liquiritin and isoliquiritigenin had hepato-protective activity on the cell model.The minimum effective concentration of glycyrrhizin was 0.001 6 μmol·L-1.The hepato-protective maximum efficacy of glycyrrhizin was 47.0% at the concentration of 0.2 μmol·L-1.The minimum effective concentration of liquiritin was 0.4 μmol·L-1.The hepato-protective maximum efficacy of liquiritin was 22.9 % at the concentration of 2 μmol·L-1.The minimum effective concentration of isoliquritigenin was 0.4 μmol·L-1.The hepato-protective maximum efficacy of isoliquritigenin was 72.6%at the concentration of 250 μmol·L-1.Conclusion:The new HL-7702 cells injury model induced by acetaminophen could be applied on studying the hepato-protective effect of drugs.The hepato-protective activity of licorice has connection with not only glycyrrhizin

liquiritin but also isoliquritigenin.Therefore

it is not enough to treat glycyrrhizin

liquiritin as the quality evaluation index for licorice.

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CHEN Yun-hua

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School of Pharmacy, Key Laboratory of Traditional Chinese Medicine(TCM) Resources with Southwest Characteristics, Chengdu University of TCM

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