YANG Xin-xin, WANG Wei, BAO Yong-rui, et al. Optimization of Purification Technology for Effective Material Group from Anemarrhena asphodeloides for Damp Arthralgia[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(8): 7-9.
YANG Xin-xin, WANG Wei, BAO Yong-rui, et al. Optimization of Purification Technology for Effective Material Group from Anemarrhena asphodeloides for Damp Arthralgia[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(8): 7-9. DOI: 10.13422/j.cnki.syfjx.2012.08.003.
Optimization of Purification Technology for Effective Material Group from Anemarrhena asphodeloides for Damp Arthralgia
摘要
目的:优选知母治疗湿热痹症有效物质组纯化工艺参数。方法:以芒果苷为指标
HPLC测定含量
对8种不同类型树脂进行考察
优选最佳树脂型号及其纯化条件。结果:确定最佳纯化条件为HPD-400型大孔树脂
加2 BV提取液进行吸附
分别用3 BV水
6 BV 30%乙醇洗脱。纯化后芒果苷纯度比原药液中含量提高6.6倍
总固体物减少87.3%。结论:纯化后药液中多糖等杂质明显减少
以芒果苷为代表的皂苷类成分得以较好的保留
为知母治疗湿热痹证研究提供前期研究基础。
Abstract
Objective:To optimize purification technology parameters of effective material group from Anemarrhena asphodeloides for damp arthralgia in clinical.Method: With mangiferin as index
eight different types macroporous resins were investigated
optimum resin and its purification conditions were optimized.Result: Optimum macroporous resin was HPD-400 resin
and its purification conditions were as follows:added 2 BV extract liquid to adsorption
then eluted by 3 BV water
and eluted with 6 BV 30% ethanol.After purified
purity of mangiferin increased 6.6 times as original liquid
total solids reduced 87.3%.Conclusion: After purified
polysaccharide and other impurities reduced significantly in medicine liquid
mangiferin(as represent of saponins) was preserved better
this study provided preliminary research base for treatment of damp arthralgia with A.asphodeloides.