Enzymatic Process of Ethanol Extract from Periplaneta americana by Papain

LUO Ting-shun; MA Fang-fang; GAO Meng-ting; WU Shao-hui; LIU Guang-ming; ZHAO Yu; ZHANG Cheng-gui

doi:10.13422/j.cnki.syfjx.2012.08.004

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Enzymatic Process of Ethanol Extract from Periplaneta americana by Papain

更新时间：2024-09-23

- Enzymatic Process of Ethanol Extract from Periplaneta americana by Papain
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 8, Pages: 15-17(2012)
- 作者机构：
  
  大理学院药学与化学学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.08.004
  CLC： TQ461
- Published：2012
- 稿件说明：
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[1]罗廷顺,马芳芳,高孟婷,吴少辉,刘光明,赵昱,张成桂.美洲大蠊醇提取物的木瓜蛋白酶酶解工艺[J].中国实验方剂学杂志,2012,18(08):15-17.

LUO Ting-shun, MA Fang-fang, GAO Meng-ting, et al. Enzymatic Process of Ethanol Extract from Periplaneta americana by Papain[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(8): 15-17.
[1]罗廷顺,马芳芳,高孟婷,吴少辉,刘光明,赵昱,张成桂.美洲大蠊醇提取物的木瓜蛋白酶酶解工艺[J].中国实验方剂学杂志,2012,18(08):15-17. DOI： 10.13422/j.cnki.syfjx.2012.08.004.

LUO Ting-shun, MA Fang-fang, GAO Meng-ting, et al. Enzymatic Process of Ethanol Extract from Periplaneta americana by Papain[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(8): 15-17. DOI： 10.13422/j.cnki.syfjx.2012.08.004.

摘要

目的:优选木瓜蛋白酶对美洲大蠊醇提取物的酶解工艺。方法:以水解度为指标

通过单因素和正交试验

分别考察酶解温度、酶解时间、pH、加酶量、底物浓度对木瓜蛋白酶酶解美洲大蠊醇提取物的影响。结果:木瓜蛋白酶酶解美洲大蠊醇提取物的最佳工艺条件为酶解温度55℃

时间3 h

pH 7.0

加酶量8 400 U·mg-1

底物浓度0.5%。在此条件下

水解度可达9.87%。酶解后的样品相对分子质量变小

相对分子质量分布变宽。结论:优选方法稳定

操作简便

为进一步研究美洲大蠊提供依据和基础。

Abstract

Objective:To study on enzymatic technology of ethanol extract from Periplaneta americana by papain and screen out optimum enzymatic technology.Method: Taking degree of hydrolysis as index

effect of enzymatic temperature

enzymatic time

the amount of papain and substrate concentration on papain were investigated by single factor experiment and orthogonal test.Result: Optimum enzymatic technology conditions of papain was determined as follows:enzymatic temperature 55 ℃

enzymatic time 3 h

pH 7.0

the amount of papain 8 400 U·mg-1

substrate concentration 0.5%.Under these conditions

degree of hydrolysis could reach to 9.87%.Molecular weight of sample were smaller and molecular weight distribution were wider after enzymolysis.Conclusion: This optimized method was stable

easy to operate

and provided basis and foundation to further study of P.americana.

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Related Author

LUO Ting-shun

MA Fang-fang

GAO Meng-ting

WU Shao-hui

LIU Guang-ming

ZHAO Yu

ZHANG Cheng-gui

SUN Jian-ning

Related Institution

Beijing University of Chinese Medicine

Beijing Hongyuan Aoda Biotechnology Development Co.Ltd

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