LI Xiao-kun, YU Lei, HAO Peng-fei, et al. Enzyme Extraction Process of Hypericin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(8): 46-49.
LI Xiao-kun, YU Lei, HAO Peng-fei, et al. Enzyme Extraction Process of Hypericin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(8): 46-49. DOI: 10.13422/j.cnki.syfjx.2012.08.012.
Enzyme Extraction Process of Hypericin
摘要
目的:探讨贯叶金丝桃中金丝桃素的最佳酶提取工艺。方法:以高效液相色谱法测定金丝桃素含量为指标
从纤维素酶、果胶酶、木聚糖酶、β-葡聚糖酶及复合酶SPE-007A中筛选出最优酶
采用单因素试验和正交试验优选提取工艺条件。结果:最优酶为纤维素酶
优选酶解工艺为酶用量2.0%
pH 4.8
温度50℃
酶解时间5 h
浸提倍数8倍。结论:该优选工艺高效、稳定。
Abstract
Objective:To investigate optimum enzyme extraction technology of hypericin from Hypericum perforatum.Method: With the content of hypericin was determined by HPLC as index
optimum enzyme was selected from cellulase
pectinase
xylanase
β-glucanase and enzyme SPE-007A
extraction technology conditions was optimized by single factor test and orthogonal test.Result: The best enzyme was cellulase
optimum extraction process was as follows:the amount of enzyme 2.0%
pH 4.8
extraction temperature 50 ℃
hydrolysis time 5 h
liquid-solid ratio 8 times.Conclusion: This optimized technology was efficient and stable.