Studies on Content Determination of Macrozamin and Total Flavonoids in Rhizoma Heterosmilacis

LI Jin-ran1; ZHANG Si-ju2; LI Lin1; HAI Li-na1; YUAN Xiu-zhi1; KUAI Yu-hua1; YANG Zhen-qiu1

doi:10.13422/j.cnki.syfjx.2012.08.036

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Studies on Content Determination of Macrozamin and Total Flavonoids in Rhizoma Heterosmilacis

更新时间：2024-09-23

- Studies on Content Determination of Macrozamin and Total Flavonoids in Rhizoma Heterosmilacis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 8, Pages: 68-71(2012)
- 作者机构：
  
  1. 北京振东光明药物研究院有限公司
  2. 中国中医科学院中药研究所
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.08.036
  CLC： R284.1
- Published：2012
- 稿件说明：
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[1]李进冉,张思巨,李琳,海丽娜,苑秀芝,蒯玉花,仰振球.白土苓中大泽明素与总黄酮的含量测定[J].中国实验方剂学杂志,2012,18(08):68-71.

LI Jin-ran1, ZHANG Si-ju2, LI Lin1, et al. Studies on Content Determination of Macrozamin and Total Flavonoids in Rhizoma Heterosmilacis[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(8): 68-71.
[1]李进冉,张思巨,李琳,海丽娜,苑秀芝,蒯玉花,仰振球.白土苓中大泽明素与总黄酮的含量测定[J].中国实验方剂学杂志,2012,18(08):68-71. DOI： 10.13422/j.cnki.syfjx.2012.08.036.

LI Jin-ran1, ZHANG Si-ju2, LI Lin1, et al. Studies on Content Determination of Macrozamin and Total Flavonoids in Rhizoma Heterosmilacis[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(8): 68-71. DOI： 10.13422/j.cnki.syfjx.2012.08.036.

摘要

目的:考察白土苓药材中大泽明素、总黄酮的含量。方法:分别采用高效液相法和紫外-可见分光光度法对白土苓药材中的大泽明素和总黄酮进行了含量测定。结果:白土苓药材中大泽明素的平均含量为0.48%

在0.148 8～0.744 1μg呈良好的线性关系（r=0.999 4）

回收率为101.07%

RSD 1.27%;总黄酮的平均含量为0.52%

橙皮苷在3.708～25.956 mg·L-1与吸光度呈良好的线性关系（r=0.999 9）

回收率为99.44%

RSD 1.46%。结论:该方法准确、简便

为科学评价白土苓药材的质量提供依据。

Abstract

Objective:To determine the content of macrozamin and total flavonoids in Rhizoma Heterosmilacis.Method: The content of macrozamin and total flavonoids was determined separately by HPLC and ultraviolet spectrophotometry（UV）.Result: The average content of macrozamin in Rhizoma Heterosmilacis was 0.48%.The calibration curve of macrozamin showed good linearity in the range of 0.148 8-0.744 1 μg（r=0.999 4）

with recovery rate 101.07% and RSD 1.27%.The average content of total flavonoids was 0.52%.The calibration curve of hesperidin showed good linearity in the range of 3.708-25.956 mg·L-1（r=0.999 9）

with recovery rate 99.44% and RSD 1.46%.Conclusion: The methods were approved accurate and simple.They could be used to scientifically evaluate Rhizoma Heterosmilacis.

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Related Author

LI Jin-ran

ZHANG Si-ju

LI Lin

HAI Li-na

YUAN Xiu-zhi

KUAI Yu-hua

YANG Zhen-qiu

GUANG Qin

Related Institution

Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences and Peking Union Medical College

School of Life Sciences， Tsinghua University

Center of Pharmacy R&D，XinJiang Huachun Bio-Pharmaceutical Co. Ltd.

Pharmacy Technology Center，Tsinghua University

School of Pharmacy， Henan University of Chinese Medicine

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