HPLC Determination of Palmatine Hydrochloride in Tinospora hainanensis

WU Li-yuan1; ZHANG Peng-wei2; DONG Lin2; LIU Ming-sheng3; YUAN Zhong-wen1; ZHOU Yu-bin1

doi:10.13422/j.cnki.syfjx.2012.08.038

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HPLC Determination of Palmatine Hydrochloride in Tinospora hainanensis

更新时间：2024-09-23

- HPLC Determination of Palmatine Hydrochloride in Tinospora hainanensis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 8, Pages: 75-77(2012)
- 作者机构：
  
  1. 广州中医药大学中药学院
  2. 海南医学院药学院
  3. 海南省南药黎药研究院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.08.038
  CLC： R284.1
- Published：2012
- 稿件说明：
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[1]吴丽媛,张鹏威,董琳,刘明生,袁中文,周郁斌.HPLC测定海南青牛胆中盐酸巴马汀的含量[J].中国实验方剂学杂志,2012,18(08):75-77.

WU Li-yuan1, ZHANG Peng-wei2, DONG Lin2, et al. HPLC Determination of Palmatine Hydrochloride in Tinospora hainanensis[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(8): 75-77.
[1]吴丽媛,张鹏威,董琳,刘明生,袁中文,周郁斌.HPLC测定海南青牛胆中盐酸巴马汀的含量[J].中国实验方剂学杂志,2012,18(08):75-77. DOI： 10.13422/j.cnki.syfjx.2012.08.038.

WU Li-yuan1, ZHANG Peng-wei2, DONG Lin2, et al. HPLC Determination of Palmatine Hydrochloride in Tinospora hainanensis[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(8): 75-77. DOI： 10.13422/j.cnki.syfjx.2012.08.038.

摘要

目的:建立海南青牛胆药材中盐酸巴马汀的HPLC含量测定方法。方法:采用高效液相色谱法

Agilent TC-C18（4.6 mm×250 mm

5μm）柱

流动相为乙腈-0.4%三乙胺水溶液（24∶76）

用磷酸调pH至4.0

流速1.0 mL.min-1

检测波长347 nm

柱温40℃。结果:盐酸巴马汀在线性范围0.006～0.15 mg（r=0.999 9）。平均回收率为99.8%

RSD 0.8%。结论:该测定方法精密度高

重复性好

可作为海南青牛胆中盐酸巴马汀的含量测定方法。

Abstract

Objective:To establish an HPLC method for determination of palmatine hydrochloride in Tinospora hainanensis.Method: HPLC method was applied on Agilent TC-C18 column（4.6 mm×250 mm

5 μm） at 40 ℃ with acetonitrile-0.4% triethylamine（24∶76）and adjusts pH with phosphoric acid to 4.0 as the mobile phase;detected wavelength was at 347 nm

and the flow rate was 1.0 mL·min-1.Result: The linear rage of palmatine chloride was 0.006-0.15 mg（r=0.999 9）.The average recovery was 99.8% and RSD was 0.8%.Conclusion: This determination method is accurate and reproducible

which can offer a suitable method for the determination of palmatine hydrochloride in T.hainanensis

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WU Li-yuan

ZHANG Peng-wei

DONG Lin

LIU Ming-sheng

YUAN Zhong-wen

ZHOU Yu-bin

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Related Institution

Department of Pharmacy,First Affiliated Hospital of PLA General Hospital

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School of Pharmaceutical Sciences， Changchun University of Chinese Medicine

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