XIE Qing-chun1, LIANG Zhao-feng1, CHEN Hui1, et al. Study on Determination of Ferulic Acid in Jiehongdieda Cataplasm[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(8): 77-79.
XIE Qing-chun1, LIANG Zhao-feng1, CHEN Hui1, et al. Study on Determination of Ferulic Acid in Jiehongdieda Cataplasm[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(8): 77-79. DOI: 10.13422/j.cnki.syfjx.2012.08.039.
Study on Determination of Ferulic Acid in Jiehongdieda Cataplasm
摘要
目的:探索HPLC测定竭红跌打凝胶膏剂中阿魏酸的含量。方法:采用Kromasil C18色谱柱(4.6 mm×250 mm
Objective:To develop a HPLC method for determination off ferulic acid in Jiehong Dieda cataplasm.Method: Kromasil C18(4.6 mm×250 mm
5 μm) column was used
acetonitrile-water-triethylamine(9∶91∶0.15
pH was adjusted to 4.0 by acetic acid) as mobile phase.The flow rate was 1.0 mL·min-1
detection wavelength at 316 nm
and column temperature at 40 ℃.Result: The linear response ranges were 0.631-20.20 mg·L-1 with regression curve A=0.794 8C+0.006 4(r=0.999 7) for ferulic acid.The average recovery of ferulic acid was 100.62%(RSD 3.08%
n=6).Conclusion: A simple
accurate and reproducible HPLC method is provided for determination of ferulic acid in Jiehong Dieda cataplasm.