XIN Hua, SHAN Yi-min, CHENG Ruo-min, et al. Simultaneously Determine the Contents of Three Components in Honeysuckle and the Red Gland Honeysuckle by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(8): 83-85.
XIN Hua, SHAN Yi-min, CHENG Ruo-min, et al. Simultaneously Determine the Contents of Three Components in Honeysuckle and the Red Gland Honeysuckle by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(8): 83-85. DOI: 10.13422/j.cnki.syfjx.2012.08.041.
Simultaneously Determine the Contents of Three Components in Honeysuckle and the Red Gland Honeysuckle by HPLC
摘要
目的:建立同时测定忍冬与红腺忍冬中绿原酸、芦丁和槲皮素的HPLC分析方法。方法:依利特Hypersil ODS(4.6 mm×250 mm
5μm)
流动相乙腈-0.05%磷酸梯度洗脱
检测波长327 nm
柱温25℃
进样10μL
流速1.0 mL·min-1。结果:线性范围内3种成分的标准曲线呈良好的线性关系
加样回收率在97%~101%
精密度和重复性的RSD均符合定量分析要求。结论:该方法准确、简便
具有良好的重复性和稳定性
可用于忍冬及红腺忍冬药材的质量控制。
Abstract
Objective:HPLC method was establisheet for simultaneous determinaton of chlorogenic acid
rutin and quercetin in honeysuckle and red gland honeysuckle.Method: The separation was performed on a Elite Hypersil ODS C18 column(4.6 mm×250 mm
5 μm).acetonitril-0.05% H3PO4 was used as the mobile phase.The detection wavelength was at 327 nm.The temperature was maintained at 25 ℃.The volume was 10 μL.The flow rate was 1.0 mL·min-1.Result: Within Linear range
the three components of the standard curve are in good linear relationship within linear range.The average recovery was 97%-101% recovery.RSD of precision and repeatability accord with quantitative analysis requirements.Conclusion: This method is accurate
simple
with good repeatability and stability and can be used for honeysuckle and red gland honeysuckle quality control.