Study on the HPLC Fingerprint of Rhizoma wenyujin Concisum

HU Guang1; CHEN Liang-mian 2; SONG Bei 2; ZHU Jing-jing 2; LIU Shou-jin1

doi:10.13422/j.cnki.syfjx.2012.08.047

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Study on the HPLC Fingerprint of Rhizoma wenyujin Concisum

更新时间：2024-09-23

- Study on the HPLC Fingerprint of Rhizoma wenyujin Concisum
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 8, Pages: 106-110(2012)
- 作者机构：
  
  1. 安徽中医学院
  2. 中国中医科学院中药研究所
  3. 中药质量控制技术国家工程实验室
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.08.047
  CLC： R284.1
- Published：2012
- 稿件说明：
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[1]户光,陈两绵,宋蓓,朱晶晶,刘守金.片姜黄HPLC指纹图谱研究[J].中国实验方剂学杂志,2012,18(08):106-110.

HU Guang1, CHEN Liang-mian 2, SONG Bei 2, et al. Study on the HPLC Fingerprint of Rhizoma wenyujin Concisum[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(8): 106-110.
[1]户光,陈两绵,宋蓓,朱晶晶,刘守金.片姜黄HPLC指纹图谱研究[J].中国实验方剂学杂志,2012,18(08):106-110. DOI： 10.13422/j.cnki.syfjx.2012.08.047.

HU Guang1, CHEN Liang-mian 2, SONG Bei 2, et al. Study on the HPLC Fingerprint of Rhizoma wenyujin Concisum[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(8): 106-110. DOI： 10.13422/j.cnki.syfjx.2012.08.047.

摘要

目的:建立不同产地片姜黄药材的指纹图谱。方法:采用HPLC

建立了16批不同产地的片姜黄药材的指纹图谱。色谱柱Kromasil 100-5C18（4.6 mm×250 mm

5μm）

流动相乙腈-水溶液梯度洗脱

柱温35℃

流速0.8 mL·min-1

检测波长244 nm

提取41个色谱峰作为化学性状

分别采用相似度评价

聚类分析和主成分分析等方法

对所收集的16批样品进行系统的比较与归类。结果:建立片姜黄专属性的指纹图谱

确定了14个共有峰

将不同产地的样品分为三大类。结论:该方法重复性好

简便可靠

可用于片姜黄药材的鉴别

可以为片姜黄的质量控制和临床使用提供依据。

Abstract

Objective:To find a quality evaluation method of Concisum Rhizoma Wenyujin.Method: HPLC fingerprint profiles of sixteen samples were established.Column Kromasil 100-5C18（4.6 mm×250 mm

5 μm） was used.The mobile phase was acetonitrile and water

and the flow rate was 0.8 mL·min-1.A matrix including 41 variations（characteristic peaks area） and 16 cases（samples） was constructed for similarity evaluation

cluster analysis and principle component analysis.Result: The results showed that the collected samples had a good similarity.A specificity fingerprint chromatogram was produced and fourteen characteristic peaks were designated.Sixteen samples were divided into three groups.Conclusion: It was a reliable and available method of quality control of Rhizoma wenyujin concisum and it was necessary for the safe and practicable use of this herbal medicine.

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