LI Qi-yu1, ZHANG Gui-jun1, LI Su-li2, et al. Production of Polysaccharide Components from Faeces Bombycis Gui and Analysis by GC-MS[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(8): 113-115.
LI Qi-yu1, ZHANG Gui-jun1, LI Su-li2, et al. Production of Polysaccharide Components from Faeces Bombycis Gui and Analysis by GC-MS[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(8): 113-115. DOI: 10.13422/j.cnki.syfjx.2012.08.049.
Production of Polysaccharide Components from Faeces Bombycis Gui and Analysis by GC-MS
Objective:To produce the polysaccharide components from Faeces Bombycis Gui and analyze its composition.Method: Polysaccharide component of Faeces Bombycis Gui was extracted by the methods of water extracting-alcohol precipitating after degreasing
and then removed protein with 10% content of trichloroa cetic acid(TCA)
purified with AB-8 macroreticular resin and decolored by active carbon.Finally
the polysaccharide component after derivatization was analyzed by gas chromatography-mass.Result: The yield of crude polysaccharide could attain 23.5%.The polysaccharide of Faeces Bombycis Gui was composed of ribose
xylose
rhamnose
galactose
glucopyranose and arabinose.The proportion of it was 1.97∶3.14∶0.58∶0.92∶5.62.Conclusion: The GC-MS method could analyze the polysaccharide components of Faeces Bombycis Gui effectively.