CHEN Qiao-qiao1, WAN Qin2, WANG Zhen-zhong2, et al. A Method for Determination of the Uronic Acids in Polysaccharides Isolated from Panax ginseng[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(8): 121-124.
CHEN Qiao-qiao1, WAN Qin2, WANG Zhen-zhong2, et al. A Method for Determination of the Uronic Acids in Polysaccharides Isolated from Panax ginseng[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(8): 121-124. DOI: 10.13422/j.cnki.syfjx.2012.08.051.
A Method for Determination of the Uronic Acids in Polysaccharides Isolated from Panax ginseng
摘要
目的:建立人参多糖中糖醛酸的含量测定方法。方法:将葡萄糖和半乳糖醛酸配成一系列混合糖
考察中性糖在咔唑法和间羟基联苯法中对糖醛酸测定的干扰
选择干扰较小的方法作为人参多糖中糖醛酸的含量测定方法
并对该方法的测定条件进行优选
对方法学进行考察。结果:选择间羟基连本法作为人参多糖中糖醛酸的含量测定方法
最佳测定条件为:四硼酸钠-硫酸溶液用量为6.0 mL
加热时间为12 min
间羟基联苯加入量为80μL
显色时间为40 min
测定波长为525nm。人参多糖中糖醛酸的平均含量为29.34%。结论:该方法简便、准确
为人参多糖的的质量控制提供可借鉴的分析方法。
Abstract
Objective:To establish the method for determination of the uronic acids in Polysaccharides isolated from Panax ginseng.Method: Mix the D-glucose and D-galacturonic in different proportions
and then use Carbazole method and Meta-hydroxyldiphenyl method to determine the D-galacturonic in the mixed sugar.Compare the interference of D-glucose on the determination results of D-galacturonic in this two methods.Choose the method of small disturbance as the determination method of the uronic acid in Panax ginseng Polysaccharides.Result: Using Meta-hydroxyldiphenyl method to determine the aldonic acid in Panax ginseng Polysaccharides and the average content is 29.34%.The optimized parameters were obtained as follows: the dosage of Na2B4O7-H2SO4 and m-hydroxydiphenyl was 6.0 mL and 100 μL
time for heating and color development were 12 min and 40 min respectively.The detection wavelength was 525 nm.Conclusion: Experimental results show that the method is easy and accurate
so it can be used for quality control of polysaccharides in Panax ginseng and its preparations.