Effect of Huogu Ⅱ Formula Combined with Different Meridian-guiding Herb on the Expression of Vascular Endothelial Growth Factor in Femoral Head Necrosis

KONG Xiang-ying1; TIAN Neng 1; WANG Rong-tian1; LIN Na1; LI Li2; CHEN Wei-heng2

doi:10.13422/j.cnki.syfjx.2012.08.081

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Effect of Huogu Ⅱ Formula Combined with Different Meridian-guiding Herb on the Expression of Vascular Endothelial Growth Factor in Femoral Head Necrosis

更新时间：2024-09-23

- Effect of Huogu Ⅱ Formula Combined with Different Meridian-guiding Herb on the Expression of Vascular Endothelial Growth Factor in Femoral Head Necrosis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 8, Pages: 237-241(2012)
- 作者机构：
  
  1. 中国中医科学院中药研究所
  2. 中国中医科学院望京医院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.08.081
  CLC： R285
- Published：2012
- 稿件说明：
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[1]孔祥英,田能,王荣田,林娜,李莉,陈卫衡.活骨Ⅱ方加不同引经药对股骨头坏死兔股骨头内血管内皮生长因子表达的影响[J].中国实验方剂学杂志,2012,18(08):237-241.

KONG Xiang-ying1, TIAN Neng 1, WANG Rong-tian1, et al. Effect of Huogu Ⅱ Formula Combined with Different Meridian-guiding Herb on the Expression of Vascular Endothelial Growth Factor in Femoral Head Necrosis[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(8): 237-241.
[1]孔祥英,田能,王荣田,林娜,李莉,陈卫衡.活骨Ⅱ方加不同引经药对股骨头坏死兔股骨头内血管内皮生长因子表达的影响[J].中国实验方剂学杂志,2012,18(08):237-241. DOI： 10.13422/j.cnki.syfjx.2012.08.081.

KONG Xiang-ying1, TIAN Neng 1, WANG Rong-tian1, et al. Effect of Huogu Ⅱ Formula Combined with Different Meridian-guiding Herb on the Expression of Vascular Endothelial Growth Factor in Femoral Head Necrosis[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(8): 237-241. DOI： 10.13422/j.cnki.syfjx.2012.08.081.

摘要

目的:观察比较活骨Ⅱ方配伍不同引经药对股骨头坏死（ONFH）兔股骨头内血管内皮生长因子（VEGF）表达的影响

探讨其防治ONFH的作用机制。方法:84只兔经液氮冷冻法建立股骨头坏死模型后

随机分为以下6组:模型组活骨Ⅱ方组、活骨Ⅱ方加不同引经药组（牛膝、独活、细辛和桔梗）

另14只设为伪手术组。造模后中药组立即分别给予活骨Ⅱ方（6 g·kg-1·d-1）、活骨Ⅱ方（6 g·kg-1·d-1）加引经药（牛膝0.75 g·kg-1·d-1

细辛0.15 g·kg-1·d-1

独活0.75 g·kg-1·d-1

桔梗0.75 g·kg-1·d-1

分别相当于临床等效量）灌胃

连续4周;模型和伪手术组给予生理盐水灌胃

所有动物均皮下注射重组人粒细胞集落刺激因子（rhG-CSF）30μg·kg-1·d-1

连续7 d。实验2周和4周时取左侧股骨头

分别行苏木精-伊红（HE）染色观察其组织病理学改变和墨汁灌注染色观察股骨头内血管形态变化

并经免疫组织化学法检测股骨头内VEGF的表达水平。结果:与伪手术组相比

模型组实验2周和4周时股骨头空骨陷窝率显著增多

血管面积及VEGF表达明显减少（P<0.01或P<0.05）。活骨Ⅱ方能显著降低股骨头空骨陷窝率

并提高血管面积及VEGF的表达（P<0.01或P<0.05）。与活骨Ⅱ组相比

牛膝组4周时空骨陷窝率显著减少

血管面积明显增多（P<0.01或P<0.05）

2周和4周VEGF表达均明显增多（P<0.01）;桔梗组4周时VEGF表达显著降低;细辛和独活组无显著变化。结论:引经药牛膝具有进一步提高活骨Ⅱ方上调股骨头内VEGF表达的作用

这可能是其有效改善股骨头血循环、防止坏死的机制之一。

Abstract

Objective:To investigate the effect of Huogu Ⅱ formula and its combination with different meridian-guiding herb on the expression of vascular endothelial growth factor（VEGF） in the femoral head of rabbits with osteonecrosis of the femoral head（ONFH）

as well as to explore the mechanism of prevention and treatment for ONFH.Method: The animal model of ONFH was established by liquid nitrogen frozen in the rabbit left hind leg.Ninety-eight.Japanese white rabbits were randomized to sham-operated group

model group

Huogu Ⅱ group and Huogu Ⅱ plus achyranthes root

Pubescent angelica

asarum and Balloonflower respectively

evenly 14 in each group.During the course of ONFH animal model establishment

all the rabbits were subcutaneously injected with recombinant human granulocyte colony-stimulating factor（rhG-CSF）（30 μg·kg-1·d-1

for continuous 7 days）.Meanwhile normal saline and decoction of the formulae were administrated by gavage respectively.Materials were drawn at the second and forth week after model establishment Histopathological changes were observed by HE staining

vascular morphology was observed by ink perfusion

VEGF was measured by immunohistochemical assay in femoral heads of the left hind leg.Result: Compared with sham-operated group

the ratio of empty lacuna increased sharply

vessel area and VEGF levels decreased obviously in the model group.But in comparison with the model group

the empty lacuna ratio of Huogu II group decreased obviously

vessel area

VEGF increased significantly.Compared with Huogu II group

the empty lacuna ratio decreased and vessel area increased significantly at the forth week

and the expression of VEGF increased both at the second and forth week in the achyranthes root group.As for the balloonflower group

empty lacuna ratio showed the increase potention trend

and the number of VEGF-positive cells decreased obvious at the forth week.There were no significant changes in Asarum and Pubescent angelica combination group.Conclusion: The medicinal guide achyranthes root could enhance the effect of Huogu Ⅱ formula to increase the expression of VEGF in the ONFH animal model.It may contribute to the preventive and therapeutic effect for ONFH.

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Related Author

KONG Xiang-ying

TIAN Neng

WANG Rong-tian

LIN Na

LI Li

CHEN Wei-heng

LIU Quan

HE Yiqing

Related Institution

Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases， Hunan University of Chinese Medicine

Hunan University of Chinese Medicine

MOE-Anhui Joint Collaborative Innovation Center for Quality Improvement of Anhui Genuine Chinese Medicinal Materials

Anhui Province Key Laboratory of Chinese Medicinal Formula

School of Pharmacy，Anhui University of Chinese Medicine

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