HPLC Fingerprint for Commercial Glycyrrhizae Radix from North-west China

LI Cheng-yi; MA Yan-ru; LI Yue-feng; WANG Ming-wei

doi:10.13422/j.cnki.syfjx.2012.09.021

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HPLC Fingerprint for Commercial Glycyrrhizae Radix from North-west China

更新时间：2024-09-23

- HPLC Fingerprint for Commercial Glycyrrhizae Radix from North-west China
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 9, Pages: 129-132(2012)
- 作者机构：
  
  甘肃中医学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.09.021
  CLC： R284.1
- Published：2012
- 稿件说明：
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[1]李成义,马艳茹,李越峰,王明伟.西北地区商品甘草HPLC指纹图谱[J].中国实验方剂学杂志,2012,18(09):129-132.

LI Cheng-yi, MA Yan-ru, LI Yue-feng, et al. HPLC Fingerprint for Commercial Glycyrrhizae Radix from North-west China[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 129-132.
[1]李成义,马艳茹,李越峰,王明伟.西北地区商品甘草HPLC指纹图谱[J].中国实验方剂学杂志,2012,18(09):129-132. DOI： 10.13422/j.cnki.syfjx.2012.09.021.

LI Cheng-yi, MA Yan-ru, LI Yue-feng, et al. HPLC Fingerprint for Commercial Glycyrrhizae Radix from North-west China[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 129-132. DOI： 10.13422/j.cnki.syfjx.2012.09.021.

摘要

目的:研究西北地区商品甘草的高效液相指纹图谱

为建立商品甘草的行业质量标准提供依据。方法:采用高效液相色谱法

选用ZORBAX Edipse XDB-C18柱（4.60 mm×250 mm

5μm）

柱温25℃

以乙腈-0.05%磷酸溶液为流动相

检测波长237 nm

进行梯度洗脱

生成图谱。使用"中药色谱指纹图谱相似度评价系统2004A"和SPSS软件建立HPLC对照指纹图谱并进行聚类分析。结果:建立了西北地区商品甘草的高效液相指纹图谱

方法学验证符合要求。16批商品甘草药材的指纹图谱有10个特征共有峰

与生成对照图谱比较相似度均>0.904

通过聚类分析将16批药材聚为4类。结论:该方法稳定、可靠

本研究首次采用HPLC指纹图谱比较西北地区不同等级的商品甘草

为西北地区商品甘草的质量考察、等级划分提供有益参考。

Abstract

Objective: Research the HPLC fingerprint of the pieces of commercial Glycyrrhizae Radix from the north-west China

and establish the quality standards of commercial Glycyrrhizae Radix to provide a basis for the industry.Method: High performance liquid chromatography was used for the soparation on.Choice ZORBAX Edipse XDB-C18 column（4.60 mm×250 mm

5 μm）

column temperature was maintained at 25 ℃

with acetonitrile-0.05% phosphoric acid was selected as mobile phase by gradient elution

the detection wavelength was set at 237 nm

the gradient elution were adopted.Using the ’chromatographic fingerprint evaluation system 2004A’ and SPSS software was used to establish HPLC fingerprint comparison and cluster analysis.Result: Established the HPLC fingerprint of the pieces of commercial Glycyrrhizae Radix from the north-west area China was established

and the results of methodological study met the technical requirements for fingerprinting.Ten common peaks were found in the fingerprint of Sixteen batches of commercial Glycyrrhizae Radix

the similarity was greater than 0.904

and four groups were divided by cluster analysis

together into four groups.Conclusion: This is the first use of HPLC fingerprint to compare different levels of commercial Glycyrrhizae Radix from the north-west China.The HPLC method is stable and reliable to provide a scientific basis of quality inspection and classification for the commercial Glycyrrhizae Radix.

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XIE Wei

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Related Institution

Key Laboratory of Modern Preparation of Traditional Chinese Medicine （TCM），Ministry of Education，Jiangxi University of TCM

Kunming Municipal Hospital of Traditional Chinese Medicine

Yunnan Provincial Key Laboratory of Research on External Drug Delivery System and Preparation Technology in University，Yunnan University of Chinese Medicine

College of Pharmacy and Chemistry, Key Laboratory of Universities in Yunnan Province on Development of Genuine Medicinal Materials Resources in Western Yunnan, Dali University

The First People's Hospital of Yiliang

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