Objective: To develop a HPLC method to determine paeoniflorin in Paeonia delavayi var.lutea

and to investigate content of paeoniflorin in different parts of P.delavayi var.lutea.Method: The analysis was carried out at 30 ℃ on a TC-C18 column（4.6 mm×150 mm

5 μm） eluted with a mobile phase consisting of a mixture of acetonitrile-0.1% phosphoric acid（12∶88）.The flow rate was 1.0 mL·min-1;and the detected wavelength was 235 nm.Result: The calibration curve was linear within the concentration ranges of 0.40-4.00 μg.The regression equation was A=1 095.3 C+31.608（r=0.999 9）.The average recovery was 100.59%

while RSD was 1.66%.The concentration distribution of paeoniflorin in different parts of P.delavayi var.lutea is as follows: fruit>leaf>cork>cortex>stem>xylem.Conclusion: This method is simple

accurate and with good repeatability.The paeoniflorin can be selected as a quality control component in P.delavayi var.lutea.