Study on Fingerprints of Woodwardiae unigemmmata from Different Habitats by HPLC

MA Bing-zhi1; ZHU Li-ping1; JU Hai1; GAO Zeng-ping2

doi:10.13422/j.cnki.syfjx.2012.09.028

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Study on Fingerprints of Woodwardiae unigemmmata from Different Habitats by HPLC

更新时间：2024-09-23

- Study on Fingerprints of Woodwardiae unigemmmata from Different Habitats by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 9, Pages: 151-153(2012)
- 作者机构：
  
  1. 中日友好医院药学部
  2. 北京中医药大学中药学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.09.028
  CLC： R284.1
- Published：2012
- 稿件说明：
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[1]马秉智,朱立平,鞠海,高增平.不同产地单芽狗脊蕨HPLC指纹图谱[J].中国实验方剂学杂志,2012,18(09):151-153.

MA Bing-zhi1, ZHU Li-ping1, JU Hai1, et al. Study on Fingerprints of Woodwardiae unigemmmata from Different Habitats by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 151-153.
[1]马秉智,朱立平,鞠海,高增平.不同产地单芽狗脊蕨HPLC指纹图谱[J].中国实验方剂学杂志,2012,18(09):151-153. DOI： 10.13422/j.cnki.syfjx.2012.09.028.

MA Bing-zhi1, ZHU Li-ping1, JU Hai1, et al. Study on Fingerprints of Woodwardiae unigemmmata from Different Habitats by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 151-153. DOI： 10.13422/j.cnki.syfjx.2012.09.028.

摘要

目的:建立不同产地单芽狗脊蕨HPLC指纹图谱测定方法。方法:采用HPLC测定了9个不同产地单芽狗脊蕨样品。色谱条件为DiamonsilTMC18色谱柱（4.6 mm×200 mm

5μm）;流动相A相为乙腈

B相为水（梯度洗脱0～60 min 22%A→100%A）;检测波长265 nm;流速1.0 mL·min-1;柱温25℃。结果:建立了不同产地单芽狗脊蕨的HPLC指纹图谱

并进行了相似度比较

进一步对其中的山柰素-3-O-α-L-鼠李糖基-7-O-α-L-鼠李糖苷、山柰素-3-O-α-L-（4-O-乙酰基）鼠李糖基-7-O-α-L-鼠李糖苷进行了归属。结论:该法操作简便、重复性好

可用于单芽狗脊蕨质量控制。

Abstract

Objective: To establish the fingerprints of Woodwardiae unigemmmata from different habitats by HPLC.Method: HPLC was applied to detect W.unigemmmata samples from 9 different habitats with a DiamonsilTMC18 column by acetonitrile A-water B as mobile phase（gradient elute: 0-60 min

22%-100%A）.The flow rate was 1.0 mL·min-1.The column temperature was at 25 ℃ and the UV detection wavelength was set at 265 nm.Result: The HPLC fingerprints of W.unigemmmata from different habitats were established and the correlation coefficients of them were calculated.Furthermore

two peaks in HPLC chromatogram were identified as kaempferol-3-O-α-L-rhamnopyranoside-7-O-α-L-rhamnopyranoside

kaempferol-3-O-α-L-（4-O-acetyl）-rhamnopyranoside-7-O-α-L-rhamnopyran-oside respectively.Conclusion: The method is simple

reproducible and can be used for quality control of W.unigemmmata.

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Related Institution

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