Study on Extraction,Isolation and Antitumor Activity of Betulinic Acid from Caulis Marsdeniae tenocissimae

NI Chong; PEI Zhi-dong; ZHANG Jia-xiao; LI Chun-xue; ZHANG Hui; KANG Ting-guo

doi:10.13422/j.cnki.syfjx.2012.09.034

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Study on Extraction,Isolation and Antitumor Activity of Betulinic Acid from Caulis Marsdeniae tenocissimae

更新时间：2024-09-23

- Study on Extraction,Isolation and Antitumor Activity of Betulinic Acid from Caulis Marsdeniae tenocissimae
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 9, Pages: 172-175(2012)
- 作者机构：
  
  辽宁中医药大学药学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.09.034
  CLC： R284;R285
- Published：2012
- 稿件说明：
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[1]倪冲,裴志东,张镓小,李春雪,张慧,康廷国.乌骨藤中白桦酯酸的提取分离及抗肿瘤活性[J].中国实验方剂学杂志,2012,18(09):172-175.

NI Chong, PEI Zhi-dong, ZHANG Jia-xiao, et al. Study on Extraction,Isolation and Antitumor Activity of Betulinic Acid from Caulis Marsdeniae tenocissimae[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 172-175.
[1]倪冲,裴志东,张镓小,李春雪,张慧,康廷国.乌骨藤中白桦酯酸的提取分离及抗肿瘤活性[J].中国实验方剂学杂志,2012,18(09):172-175. DOI： 10.13422/j.cnki.syfjx.2012.09.034.

NI Chong, PEI Zhi-dong, ZHANG Jia-xiao, et al. Study on Extraction,Isolation and Antitumor Activity of Betulinic Acid from Caulis Marsdeniae tenocissimae[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 172-175. DOI： 10.13422/j.cnki.syfjx.2012.09.034.

摘要

目的:对乌骨藤中白桦酯酸进行提取分离

并探讨其抗肿瘤活性。方法:采用硅胶柱色谱法、Sephadex LH-20凝胶柱色谱法及半制备高效液相色谱法

对白桦酯酸进行提取分离

并采用结晶紫法

以细胞死亡率为检测指标

观察白桦酯酸在不同质量浓度1～500 mg·L-1情况下

对细胞密度为1×104/孔的4种肿瘤细胞分别作用12

48 h的生长抑制作用。结果:白桦酯酸对人宫颈癌细胞HeLa、人胃癌细胞MGC、人乳腺癌细胞MCF-7具有很好的增殖抑制作用

并呈剂量依赖效应

在质量浓度为500 mg·L-1作用48 h时

对Hela细胞及MGC细胞的死亡率分别为77.9%

81.7%

质量浓度为100 mg·L-1作用48 h时

对MCF-7的细胞死亡率为74.1%

但对人乳腺导管癌细胞（ZR株）作用不显著。结论:白桦酯酸对HeLa

MGC

MCF-7肿瘤细胞具有抗肿瘤活性

对ZR株肿瘤细胞作用不明显。

Abstract

Objective: Extraction and isolation of betulinic acid from Marsdenia tenacissima and investigate the anti-tumor activity were investigated.Method: By using silica gel column chromatography

Sephadex LH-20 chromatography and semi-preparative reversed phase liquid chromatograph

extraction and isolation of betulinic acid

and MTT assay was applied in demonstrating effect on antitumor effect in four tumor cells with different concentrations and at different action time.Result:The growth of cervical cancer cells HeLa

gastric cancer cells MGC

breast cancer MCF-7 was significantly inhibited by betulinic acid with a manner of dose-dependence.After 48 h

the cell death rate of HeLa and MGC was 77.9%

81.7%

respectively.When the concentration of the sample was 500 mg·L-1

the cell death rate of MCF-7 was 74.1%

when the concentration of the sample was 100 mg·L-1

the effect on ducal carcinoma cells was not significant.Conclusion: Betulinic acid has an anti-tumor activity on HeLa

MGC

MCF-7.

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Related Author

NI Chong

PEI Zhi-dong

ZHANG Jia-xiao

LI Chun-xue

ZHANG Hui

KANG Ting-guo

CHEN Lanpin

TAN Feng

Related Institution

The Affiliated Hospital of Nanjing University of Chinese Medicine

Jiangsu Collaborative Innovation Center of Traditional Chinese Medicine for Tumor Prevention and Treatment

The First School of Clinical Medicine of Nanjing University of Chinese Medicine

School of Clinical Medicine of Nanjing University of Chinese Medicine

Guang'anmen Hospital， China Academy of Chinese Medical Sciences

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