Effect of Zuogui Wan on Follicular Development and Ovarian Angiogenesis in Rats

DUAN Heng; ZHOU Ying

doi:10.13422/j.cnki.syfjx.2012.09.042

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Effect of Zuogui Wan on Follicular Development and Ovarian Angiogenesis in Rats

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- Effect of Zuogui Wan on Follicular Development and Ovarian Angiogenesis in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 9, Pages: 206-209(2012)
- 作者机构：
  
  重庆医科大学中医药学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.09.042
  CLC： R285.5
- Published：2012
- 稿件说明：
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[1]段恒,周滢.左归丸对大鼠卵泡发育与卵巢血管生成的影响[J].中国实验方剂学杂志,2012,18(09):206-209.

DUAN Heng, ZHOU Ying. Effect of Zuogui Wan on Follicular Development and Ovarian Angiogenesis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 206-209.
[1]段恒,周滢.左归丸对大鼠卵泡发育与卵巢血管生成的影响[J].中国实验方剂学杂志,2012,18(09):206-209. DOI： 10.13422/j.cnki.syfjx.2012.09.042.

DUAN Heng, ZHOU Ying. Effect of Zuogui Wan on Follicular Development and Ovarian Angiogenesis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 206-209. DOI： 10.13422/j.cnki.syfjx.2012.09.042.

摘要

目的:探讨左归丸对大鼠卵巢卵泡发育与血管生成的影响。方法:将SD大鼠（160～170 g）随机分为空白对照组（A组）、结合雌激素组（B组）、左归丸成药低（C组）、高剂量组（D组）、左归丸浸膏低（E组）、高剂量组（F组）

各组n=10。分别灌服蒸馏水20 g·kg-1、结合雌激素混悬液65μg·kg-1、左归丸混悬液1.875

5.625 g·kg-1、左归丸浸膏相当于生药11

33 g·kg-1

每日1次

连续15 d。计数大鼠卵巢各级卵泡及黄体;计算生殖器官（卵巢、子宫及阴道）脏器指数;采取酶免疫分析法测定血清雌二醇（E2）水平。将大鼠随机分为空白对照组、结合雌激素组（65μg·kg-1）、左归丸成药组（5.625 g·kg-1）、左归丸浸膏组（相当于生药33 g·kg-1）

各组n=6。应用墨汁灌注法观察卵巢血管。结果:D

B组均可增加大鼠窦状卵泡数（21.70±10.40

15.60±2.22

15.60±8.04）个及卵泡总数（32.10±11.57

25.30±5.06

29.10±18.62）个

B～F组均可增加大鼠卵巢指数（0.68±0.11

0.55±0.05

0.68±0.08

0.61±0.10

0.68±0.03）

D～F

B组均可提高大鼠血清E2水平（86.92±5.05

68.98±9.19

87.16±10.37

87.39±12.81）ng·L-1

均P<0.01或P<0.05。结合雌激素组、左归丸成药组、左归丸浸膏组的卵巢血管密度和管腔明显增大。结合雌激素组、左归丸浸膏组组可增加卵巢小血管数（13.33±2.50

13.17±3.87）个

结合雌激素组、左归丸成药组、左归丸浸膏组均可增加大鼠卵巢大血管数（33.00±5.44

34.33±8.59

35.83±7.63）个及血管总数（46.33±7.82

45.00±10.84

49.33±11.11）个

P<0.05或P<0.01。结论:左归丸可促进160～170 g大鼠卵泡发育和卵巢血管生成

其促卵泡发育作用与提高雌激素水平、增加雌激素样效应及增加卵巢血管数有关。左归丸浸膏较成药丸剂作用更明显。

Abstract

Objective:To study the effect of Zuogui Wan on follicular development and ovarian angiogenesis in the rats.Method:The SD rats（160-170 g） were divided into 6 groups: control group（group A）

conjugated estrogens group（group B）

Zuogui Wan patent medicine low dose group（group C）

Zuogui Wan patent medicine high dose group（group D）

Zuogui Wan decocted extract low dose group（group E）

Zuogui Wan decocted extract low dose group（group F）

10 rats each group.The rats were seperately administered with distilled water 20 g·kg-1

conjugated estrogens 65 μg·kg-1

Zuogui Wan suspension 1.875 g·kg-1

Zuogui Wan suspension 5.625 g·kg-1

Zuogui Wan extract equivalent to crude drug 11 g·kg-1

Zuogui Wan extract equivalent to crude drug 33 g·kg-1

for 15 d.Ovarian follicle and corpus luteum of rats were counted.Uterus indexes were counted.In addition

E2 in blood was determinated by enzyme immunoassay（EIA）.The rats were divided into 4 groups: control group

conjugated estrogens group（65 μg·kg-1）

Zuogui Wan patent medicine group（5.625 g·kg-1）

Zuogui Wan decocted extract group（equivalent to crude drug 33 g·kg-1）

6 rats each group.Ovarian micrangium was measured by perfusion of ink.Results:D

B groups increased antral follicles（21.70±10.40

15.60±2.22

15.60±8.04）and total follicles（32.10±11.57

25.30±5.06

29.10±18.62）;B-F groups increased ovary index（0.68±0.11

0.55±0.05

0.68±0.08

0.61±0.10

0.68±0.03）;D-F

B groups increased E2 level in blood of the rats（86.92±5.05

68.98±9.19

87.16±10.37

87.39±12.81）ng·L-1

all P<0.01 or P<0.05.Ovarian blood vessel density and lumen in conjugated estrogens group

Zuogui Wan patent medicine group and Zuogui Wan decocted extract group were increased significantly.Conjugated estrogens group and Zuogui Wan decocted extract group group increased ovarian small blood vessels（13.33±2.50

13.17±3.87）

conjugated estrogens group

Zuogui Wan patent medicine group and Zuogui Wan decocted extract group increased ovarian large blood vessels（33.00±5.44

34.33±8.59

35.83±7.63）and the total number of blood vessels（46.33±7.82

45.00±10.84

49.33±11.11）

P<0.05 or P<0.01.Conclusion:Zuogui Wan can improve follicular development and ovarian angiogenesis in 160-170 g rats.The effect may be related to improving the level of E2

estrogenlike effect and ovarian blood vessels.Zuogui Wan decocted extract is more effective than Zuogui Wan patent medicine.

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