FAN Kai-fang1, LIANG Xiao-dong2, LI Xiao-liang3, et al. The Effect of Sanhua Tang on Expression of ZO-1 in Brain Tissues of Cerebral Ischemia-reperfusion Rat[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 216-219.
FAN Kai-fang1, LIANG Xiao-dong2, LI Xiao-liang3, et al. The Effect of Sanhua Tang on Expression of ZO-1 in Brain Tissues of Cerebral Ischemia-reperfusion Rat[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 216-219. DOI: 10.13422/j.cnki.syfjx.2012.09.044.
The Effect of Sanhua Tang on Expression of ZO-1 in Brain Tissues of Cerebral Ischemia-reperfusion Rat
Objective:To observe the effect of Sanhua Tang on expression of zona occludens-1(ZO-1) in brain tissues of cerebral ischemia-reperfusion rat.Method:The rats were randomly divided into sham operation group
model group
low dose group of Sanhua Tang
large dose group of Sanhua Tang and nimodipine group.The middle cerebral artery was blocked with suture method to prepare cerebral ischemia reperfusion model.After cerebral ischemia for two hours and reperfusion for twenty-fourth hours
the expression of ZO-1 was detected in every brain tissues by immunohistochemical technique.Result: Compared with sham operation group
in model group expression of ZO-1 in brain tissues of cerebral ischemia-reperfusion rat was significantly decreased(P<0.01);compared with model group
expression of zo-1 in brain tissues was significantly increased in large dose group of Sanhua Tang(P<0.01).In nimodipine group the expression of zo-1 was evidently increased(P<0.05).there were no significant differences between model group and low dose of Sanhua Tang.Compared with nimodipine group
the expression of ZO-1 in brain tissues of cerebral ischemia-reperfusion rat was significantly increased in large dose group of Sanhua Tang(P<0.05).Conclusion:Sanhua Tang can protect against injure of cerebral ischemia-reperfusion in rat.