Effect of Saponin of Yulangsan on Myocardial Ischemia Reperfusion Injury in Isolated Rat Hearts

LI Jian-ling1; CHEN Zhao-ni2; LV Ji-hua2; HUANG Ren-bin2; QIN Fei-zhang2; ZHANG Shi-jun2

doi:10.13422/j.cnki.syfjx.2012.09.052

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Effect of Saponin of Yulangsan on Myocardial Ischemia Reperfusion Injury in Isolated Rat Hearts

更新时间：2024-09-23

- Effect of Saponin of Yulangsan on Myocardial Ischemia Reperfusion Injury in Isolated Rat Hearts
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 9, Pages: 250-253(2012)
- 作者机构：
  
  1. 广西医科大学第一附属医院心血管病研究所
  2. 广西医科大学药理学教研室
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.09.052
  CLC： R285.5
- Published：2012
- 稿件说明：
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[1]李健玲,陈兆霓,吕纪华,黄仁彬,覃斐章,张士军.玉郎伞皂苷对大鼠离体心脏缺血再灌注损伤的保护作用[J].中国实验方剂学杂志,2012,18(09):250-253.

LI Jian-ling1, CHEN Zhao-ni2, LV Ji-hua2, et al. Effect of Saponin of Yulangsan on Myocardial Ischemia Reperfusion Injury in Isolated Rat Hearts[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 250-253.
[1]李健玲,陈兆霓,吕纪华,黄仁彬,覃斐章,张士军.玉郎伞皂苷对大鼠离体心脏缺血再灌注损伤的保护作用[J].中国实验方剂学杂志,2012,18(09):250-253. DOI： 10.13422/j.cnki.syfjx.2012.09.052.

LI Jian-ling1, CHEN Zhao-ni2, LV Ji-hua2, et al. Effect of Saponin of Yulangsan on Myocardial Ischemia Reperfusion Injury in Isolated Rat Hearts[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 250-253. DOI： 10.13422/j.cnki.syfjx.2012.09.052.

摘要

目的:研究玉郎伞（YLS）皂苷对大鼠离体心脏缺血再灌注损伤（MI/RI）的保护作用及其作用机制。方法:40只Wistar大鼠随机分为5组（每组8只）:正常对照组

缺血-再灌注组

YLS皂苷低剂量组（2×10-2g·L-1）

YLS皂苷高剂量组（4×10-2g·L-1）

阳性对照维拉帕米组（5×10-7mol·L-1）。离体大鼠心脏采用Langendorff法灌流

停灌30 min再灌30 min造成心肌缺血-再灌注损伤模型。左心室插入水囊导管

记录YLS皂苷对血流动力学指标的影响

测定冠脉流量（CF）和冠脉流出液中肌酸激酶（CK）、肌酸激酶同工酶（CK-MB）、乳酸脱氢酶（LDH）、乳酸脱氢酶同工酶-1（LDH-1）活性以及心肌组织超氧化物歧化酶（SOD）和丙二醛（MDA）含量。结果:YLS皂苷高剂量可显著改善缺血-再灌注所致的心功能损伤

减少CK

CK-MB

LDH

LDH-1的释放和心肌组织MDA的产生

增加SOD的活性（与模型组相比

P<0.05或P<0.01）。结论:YLS皂苷对心肌缺血-再灌注损伤具有保护作用

其机制可能与清除氧自由基、减少脂质过氧化反应有关。

Abstract

Objective: To study the effect and mechanism of saponin of Yulangsan（SYLS） on myocardial ischemia reperfusion injury in isolated rat hearts.Method:Fourteen Wistar rats were randomly divided into five groups: normal group

ischemia reperfusion group

verapamil group（5×10-7 mol·L-1）

and SYLS groups（2×10-2

4×10-2 g·L-1）.Langendorff-perfusion isolated rat hearts were prepared.Myocardial ischemia reperfusion injury was induced by 30 minutes global ischemia followed by 30 minutes reperfusion.The effect of SYLS on cardiac hemodynamics was observed by MPA-CFS.The activities of creatine kinase（CK）

creatine kinase isoenzyme MB（CK-MB）

lactate dehydrogenase（LDH）

lactate dehydrogenase isozyme-1（LDH-1） in the effluent from coronary artery were measured to evaluate the injury of myocardial cells and the protective effect of SYLS.Meanwhile

the activity of superoxide dismutase（SOD） and the contents of malondialdehyde（MDA） in myocardial tissues were determined.Result: Compared with the I/R group

high dosages of SYLS could recover cardiac function

and reduce the releases of CK

CK-MB

LDH and LDH-1 from I/R rat hearts.It could increase the activity of SOD

and decrease the MDA product（P<0.05 or P<0.01）.Conclusion:SYLS may offer myocardial protective effects against ischemia reperfusion injury

which may be related to its effects of scavenging oxygen free radical

reducing lipid peroxidation.

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Related Author

LI Jian-ling

CHEN Zhao-ni

LV Ji-hua

HUANG Ren-bin

QIN Fei-zhang

ZHANG Shi-jun

Hong-yan LIU

Yu-xue XIAO

Related Institution

Changchun University of Chinese Medicine

Pharmacy of College,Shihezi University

The First Affiliated Hospital of Medical College,Shihezi University

Xinjiang Institute of Materia Medica

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