WANG Gang, HOU Zhi-fei, XIANG Hui-xin, et al. Optimization of Extraction Technology for Inula helenium[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 26-28.
WANG Gang, HOU Zhi-fei, XIANG Hui-xin, et al. Optimization of Extraction Technology for Inula helenium[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 26-28. DOI: 10.13422/j.cnki.syfjx.2012.09.061.
Optimization of Extraction Technology for Inula helenium
摘要
目的:确定祁木香的最佳提取工艺。方法:采用正交试验对祁木香提取工艺进行优选
以土木香内酯和异土木香内酯含量为指标
考察乙醇体积分数、溶剂用量、提取时间、提取次数4个因素的影响
采用HPLC进行含量测定。结果:最佳提取工艺为15倍量85%乙醇提取3次
每次1 h。结论:该优选提取工艺稳定可行、提取率高
为祁木香的提取提供科学依据。
Abstract
Objective:To determine optimum extraction technology of Inula helenium.Method:Orthogonal test was used to optimize extraction technology of I.helenium with the content of alantolactone and isoalantolactone as indexes.Effect of the concentration of ethanol
the amount of solvent
extraction time and extraction times were investigated
the content of index components were determined by HPLC.Result:Optimum extraction technology was as follows:refluxing extracted 3 times with 15 times the amount of 85% ethanol
1 h each time.Conclusion:This optimized extraction technology was stable and feasible with high extraction ratio
it could provide scientific basis for extraction of I.helenium.
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Related Author
WANG Gang
HOU Zhi-fei
XIANG Hui-xin
GU Fei-fei
ZHANG Xue-rong
QIN Ying
LIN Hong-ying
YANG Yan-fang
Related Institution
Drug Research Center for Nephropathy,Guangzhou Consun Pharmaceutical Co.Ltd
Key Laboratory of New Drug Delivery System of Chinese Meteria Medica,Jiangsu Provincial Academy of Chinese Medicine, Key Laboratory of Scientific Preparation for Clinical Effective Prescription of State Administration of Traditional Chinese Medicine
Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences
Medical School, Lijiang Culture and Tourism College