LAN Jun-feng1, LIU Kun2. Extraction of Gallic Acid from Phyllanthus urinaria by Inner Ebullition Method[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 63-65.
LAN Jun-feng1, LIU Kun2. Extraction of Gallic Acid from Phyllanthus urinaria by Inner Ebullition Method[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 63-65. DOI: 10.13422/j.cnki.syfjx.2012.09.067.
Extraction of Gallic Acid from Phyllanthus urinaria by Inner Ebullition Method
摘要
目的:优选内部沸腾法提取叶下珠没食子酸工艺条件。方法:用少量乙醇溶液为解吸剂润湿叶下珠粉末
使没食子酸充分解吸
加一定量热提取剂
使渗透到叶下珠组织内部的乙醇沸腾
强化提取过程。选取乙醇体积分数、解吸时间、乙醇用量、提取时间和提取温度等5个因素
4水平进行正交试验
采用高效液相色谱法测定没食子酸含量。结果:内部沸腾法提取叶下珠没食子酸的最佳工艺条件为1.6倍量60%乙醇解吸30 min
80℃提取15 min。结论:在最佳提取工艺条件下
叶下珠没食子酸提取得率可达0.970%
该法有较好的应用前景。
Abstract
Objective:To optimize extraction process of gallic acid from Phyllanthus urinaria by inner ebullition method.Method: With a small amount of ethanol as wetting agent to soak power of P.urinaria
gallic acid was resolved fully
added a certain amount of heat extraction agent to make ethanol boiling which penetrated into internal organization of P.Urinaria
enhanced extraction technology.Orthogonal test was used with the concentration of ethanol
resolution time
the amount of ethanol
extraction time and extraction temperature as factors
the content of gallic acid was determined by HPLC.Result: Optimized extraction technology of gallic acid from P.urinaria by inner ebullition method was as follows: resolved 30 min with 1.6 times the amount of 60% ethanol
extracted 15 min at 80 ℃.Conclusion: Under this optimized technology conditions
Extraction of Gallic Acid from by Inner Ebullition Method
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Related Author
LAN Jun-feng
LIU Kun
LAN Jun-feng
XIE Ji-yun
YU Lan
WEI Xue-jun
Chen Ming
YU Yue-sheng
Related Institution
School of Life Sciences,Beijing University of Chinese Medicine
The First Clinical Medical College,Nanjing University of Chinese Medicine
Affiliated Hospital of Nanjing University of Chinese Medicine/Jiangsu Province Hospital of Chinese Medicine
Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences
Medical School, Lijiang Culture and Tourism College