Determination for Content of Gentiopicroside,Naringin and Protosappanin B from Xuanbi Gel Plaster

LIU Yue-huan; DU Shou-ying; LU Yang; BAI Jie; WANG Yue; LI Hui-yun

doi:10.13422/j.cnki.syfjx.2012.09.069

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Determination for Content of Gentiopicroside,Naringin and Protosappanin B from Xuanbi Gel Plaster

更新时间：2024-09-23

- Determination for Content of Gentiopicroside,Naringin and Protosappanin B from Xuanbi Gel Plaster
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 9, Pages: 72-74(2012)
- 作者机构：
  
  北京中医药大学中药学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.09.069
  CLC： R286
- Published：2012
- 稿件说明：
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[1]刘月环,杜守颖,陆洋,白洁,王玥,李慧云.宣痹凝胶贴膏剂中龙胆苦苷、柚皮苷、原苏木素B含量测定[J].中国实验方剂学杂志,2012,18(09):72-74.

LIU Yue-huan, DU Shou-ying, LU Yang, et al. Determination for Content of Gentiopicroside,Naringin and Protosappanin B from Xuanbi Gel Plaster[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 72-74.
[1]刘月环,杜守颖,陆洋,白洁,王玥,李慧云.宣痹凝胶贴膏剂中龙胆苦苷、柚皮苷、原苏木素B含量测定[J].中国实验方剂学杂志,2012,18(09):72-74. DOI： 10.13422/j.cnki.syfjx.2012.09.069.

LIU Yue-huan, DU Shou-ying, LU Yang, et al. Determination for Content of Gentiopicroside,Naringin and Protosappanin B from Xuanbi Gel Plaster[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 72-74. DOI： 10.13422/j.cnki.syfjx.2012.09.069.

摘要

目的:建立宣痹凝胶膏剂中龙胆苦苷、柚皮苷、原苏木素B含量测定方法。方法:采用高效液相色谱法

MerckRP-C18色谱柱（4.6 mm×250 mm

5μm）

流动相乙腈-0.1%磷酸梯度洗脱（0～33 min

11%乙腈;33～36 min

11%乙腈～20%乙腈;36～67 min

20%乙腈）

柱温20℃

流速0.6 mL·min-1

检测波长283 nm

进样量10μL。结果:龙胆苦苷、柚皮苷、原苏木素B分别在0.014 9～0.745 0μg

0.007 1～0.355 0μg

0.002 2～0.107 5μg呈良好的线性关系

平均回收率分别为98.87%（RSD 2.19%）

103.51%（RSD 0.94%）

99.58%（RSD 2.49%）。结论:方法操作简便

准确

重复性好

可作为宣痹凝胶膏剂的质量控制。

Abstract

Objective:To establish content determination method for gentiopicroside

naringin and protosappanin B from Xuanbi gel plaster.Method:HPLC was used with Merck RP-C18 column（4.6 mm×250 mm

5 μm）

gradient eluted with mobile phase of 0.1% phosphoric acid-acetonitrile（0-33 min

11% acetonitrile;33-36 min

11% acetonitrile-20% acetonitrile;36-67 min

20% acetonitrile）

column temperature was 20 ℃

flow rate was 0.6 mL·min-1

detection wavelength 283 nm

injection volume was 10 μL.Result:The linear range of gentiopicroside

naringin and protosappanin B were 0.014 9-0.745 μg

0.007 1-0.355 μg

0.002 2-0.107 5 μg

respectively.Average recovery of these ingredients were 98.87%（RSD 2.19%）

103.51%（RSD 0.94%）

99.58%（RSD 2.49%）

respectively.Conclusion:This established method was simple

accurate and repeatable

it could be used as quality control method for Xuanbi gel plaster.

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