Analysis of IR Spectra on the Buds of Different Germplasm Resources of Lonicera Japonica

ZHANG Fang1; ZHANG Yong-qing1; YU Xiao2; KANG Yu-qiu1; ZHOU Feng-qin1; SUN Su-qin3

doi:10.13422/j.cnki.syfjx.2012.09.072

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Analysis of IR Spectra on the Buds of Different Germplasm Resources of Lonicera Japonica

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- Analysis of IR Spectra on the Buds of Different Germplasm Resources of Lonicera Japonica
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 9, Pages: 83-86(2012)
- 作者机构：
  
  1. 山东中医药大学
  2. 山东医药技师学院
  3. 清华大学化学系
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.09.072
  CLC： R284.1
- Published：2012
- 稿件说明：
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[1]张芳,张永清,于晓,康玉秋,周凤琴,孙素琴.忍冬不同种质花蕾红外光谱分析[J].中国实验方剂学杂志,2012,18(09):83-86.

ZHANG Fang1, ZHANG Yong-qing1, YU Xiao2, et al. Analysis of IR Spectra on the Buds of Different Germplasm Resources of Lonicera Japonica[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 83-86.
[1]张芳,张永清,于晓,康玉秋,周凤琴,孙素琴.忍冬不同种质花蕾红外光谱分析[J].中国实验方剂学杂志,2012,18(09):83-86. DOI： 10.13422/j.cnki.syfjx.2012.09.072.

ZHANG Fang1, ZHANG Yong-qing1, YU Xiao2, et al. Analysis of IR Spectra on the Buds of Different Germplasm Resources of Lonicera Japonica[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 83-86. DOI： 10.13422/j.cnki.syfjx.2012.09.072.

摘要

目的:分析鉴定山东产忍冬不同种质花蕾。方法:采用红外光谱三级鉴定法（红外光谱、二阶导数谱以及二维相关谱）对不同种质忍冬花蕾进行分析鉴定。结果:一维图谱上不同种质间相似度较大

均有3 371 cm-1处的O-H伸缩振动峰

2 924

2 853 cm-1处的-CH2处的伸缩振动峰

1 735 cm-1处的C=O伸缩振动峰

1 637 cm-1处的烯醚双键伸缩振动峰

1 051cm-1处的C-O伸缩振动峰

说明其主体成分相似;二阶导数红外图谱上

不同种质间在1 800～1 300 cm-1处差异明显;二维相关光谱中

不同种质间其峰强、峰形、峰位等均有较大差异。结论:利用傅立叶红外光谱三级鉴定技术可以鉴别不同种质来源的金银花药材。

Abstract

Objective: To analyze and identify the buds of different germplasm resources of Lonicera japonica Thunb.cultivated in ShanDong.Method: Multi-steps infrared macro-fingerprint method（infrared spectroscopy

secondary derivative spectroscopy and two dimensional correlation infrared spectroscopy） was used to analyze the buds of different germplasm resources of Lonicera japonica Thunb.Result: In the one-dimensional spectra

there have some similarity among different germplasm

appearing the absorption peak of-OH in 3 371 cm-1

the absorption peak of-CH2 in 2 924 cm-1 and 2 853 cm-1

the absorption peak of C=O in 1 735 cm-1

the absorption peak of =C-O in 1 637 cm-1 and the absorption peak of C-O in 1 637 cm-1.In the second derivative spectra

there have significant difference among different germplasm between 1 800 cm-1 and 1 300 cm-1.Otherwise

the 2D-IR correlation spectra also have significant differences in the absorption strength

frequency and form.Conclusion: The FTIR Analysis is effective for the identification of the buds of different germplasm resource.

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ZHANG Fang

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Related Institution

Gansu University of Chinese Medicine

Northwest Collaborative Innovation Center for Traditional Chinese Medicine Co-constructed by Gansu Province & MOE of PRC

Engineering Research Center for Evaluation， Protection and Utilization of Rare Traditional Chinese Medicine Resources

Dingxi Academy of Agricultural Sciences

School of Information Science and Engineering， Lanzhou University

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