Determ Ina Tion of Gallic Acid in Polygonum Capita Tum and Relinqing Granules by HPLC

WANG Shuang1; ZHANG Li-yan2; XIE Yu3; PAN Wen-ting2; LI Meng-lin3

doi:10.13422/j.cnki.syfjx.2012.09.080

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Determ Ina Tion of Gallic Acid in Polygonum Capita Tum and Relinqing Granules by HPLC

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- Determ Ina Tion of Gallic Acid in Polygonum Capita Tum and Relinqing Granules by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 9, Pages: 112-115(2012)
- 作者机构：
  
  1. 浙江中医药大学药学院
  2. 贵阳中医学院药学系
  3. 贵州威门药业股份有限公司
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.09.080
  CLC： R286.0
- Published：2012
- 稿件说明：
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[1]王爽,张丽艳,谢宇,潘雯婷,李孟林.HPLC测定头花蓼及制剂热淋清颗粒中没食子酸的含量[J].中国实验方剂学杂志,2012,18(09):112-115.

WANG Shuang1, ZHANG Li-yan2, XIE Yu3, et al. Determ Ina Tion of Gallic Acid in Polygonum Capita Tum and Relinqing Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 112-115.
[1]王爽,张丽艳,谢宇,潘雯婷,李孟林.HPLC测定头花蓼及制剂热淋清颗粒中没食子酸的含量[J].中国实验方剂学杂志,2012,18(09):112-115. DOI： 10.13422/j.cnki.syfjx.2012.09.080.

WANG Shuang1, ZHANG Li-yan2, XIE Yu3, et al. Determ Ina Tion of Gallic Acid in Polygonum Capita Tum and Relinqing Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 112-115. DOI： 10.13422/j.cnki.syfjx.2012.09.080.

摘要

目的:建立头花蓼及热淋清颗粒中没食子酸的含量测定方法。方法:采用反相高效液相色谱法

DiamonsilTM（钻石）C18柱（4.6 mm×250 mm

5μm）

流动相甲醇-水-N

N-二甲基甲酰胺-冰醋酸（1∶95∶3∶1）

流速1.0 mL·min-1

检测波长272 nm

柱温25℃。结果:没食子酸在0.139 6～1.396 0μg线性良好（r=0.999 97）。头花蓼平均回收率99.35%

RSD为2.61%

热淋清颗粒平均回收率97.86%

RSD为0.73%。结论:该法重复性好

专属性强

可用于控制头花蓼药材及其单方制剂热淋清颗粒的质量。为头花蓼药材规模化种植提供科学依据。

Abstract

Objective: To establish an HPLC method for determination of the contents of gallic acid in Polygonum capitatum and Relinqing granules.Method: The reserved HPLC method was achieved.Diamonsil C18 colunm（4.6 mm×250 mm

5 μm） with MeOH-H2O-DMF-HAc（1∶ 95∶ 3∶ 1） as amobile phase at flow rate of 1.0 mL·min-1and detection wavelength was at 272 nm and the column temperature was at 25 ℃.Result: The calibration curve was linear in the range of 0.139 6-1.396 0 μg（r=0.999 97）.The average recovery rate of gallic acid in P.capitatum was 99.35%（RSD 2.61%）

and was 99.35%（RSD 0.73%） in Relinqing granules.Conclusion: The method is reliable

specific and with good repeatability.It can be used for the quality control of P.capitatum and Relinqing granules and provide a science bases for the planting of polygonum cap itatum.

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Related Author

WANG Shuang

ZHANG Li-yan

XIE Yu

PAN Wen-ting

LI Meng-lin

ZHOU Tao

AI Qiang

WANG Yan-jun

Related Institution

Guiyang Collage of Traditional Chinese Medicine

School of Life Sciences，Beijing University of Chinese Medicine

The First Clinical Medical College，Nanjing University of Chinese Medicine

Affiliated Hospital of Nanjing University of Chinese Medicine/Jiangsu Province Hospital of Chinese Medicine

State Key Laboratory of Quality Evaluation of TCM，National Administration of TCM， Sichuan Academy of Chinese Medical Sciences

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