Comparison and Determination of Total Flavonoids in Different Parts of Berchemia lineata

MAO Hai-li; ZHOU De-chao; LONG Cheng-mei; YANG Yan

doi:10.13422/j.cnki.syfjx.2012.09.081

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Comparison and Determination of Total Flavonoids in Different Parts of Berchemia lineata

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- Comparison and Determination of Total Flavonoids in Different Parts of Berchemia lineata
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 9, Pages: 115-117(2012)
- 作者机构：
  
  黔南民族师范学院化学与化工系
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.09.081
  CLC： R284.1
- Published：2012
- 稿件说明：
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[1]毛海立,周德超,龙成梅,杨艳.铁包金中不同部位总黄酮含量比较[J].中国实验方剂学杂志,2012,18(09):115-117.

MAO Hai-li, ZHOU De-chao, LONG Cheng-mei, et al. Comparison and Determination of Total Flavonoids in Different Parts of Berchemia lineata[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 115-117.
[1]毛海立,周德超,龙成梅,杨艳.铁包金中不同部位总黄酮含量比较[J].中国实验方剂学杂志,2012,18(09):115-117. DOI： 10.13422/j.cnki.syfjx.2012.09.081.

MAO Hai-li, ZHOU De-chao, LONG Cheng-mei, et al. Comparison and Determination of Total Flavonoids in Different Parts of Berchemia lineata[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 115-117. DOI： 10.13422/j.cnki.syfjx.2012.09.081.

摘要

目的:考察了贵州都匀产铁包金不同部位总黄酮含量的差异。方法:以乙醇作为回流提取溶剂提取铁包金中总黄酮。以芦丁为对照品

采用紫外分光光度法测定铁包金中的总黄酮。结果:芦丁标准品溶液浓度在5～30μg·L-1与吸光度有良好线性关系

回归方程为Y=0.011 2X+0.041 9（r=0.999 1）

平均加样回收率98.56%

精密度试验RSD值为0.279%

重复性试验RSD为0.634%

稳定性试验RSD 1.49%。以此方法测定

铁包金叶和茎部中黄酮含量分别为12.3%

1.42%

叶中总黄酮含量是茎部的的8.7倍。结论:贵州都匀产铁包金叶和茎部中黄酮含量存在较大差异

叶部位具有较大的药用开发价值和应用前景。

Abstract

Objective: The content distribution of total flavonoids in different parts of Berchemia lineate from city of DuYun in Guizhou was investigated.Method:Flavonids in different parts of Berchemia Lineate（L）DC.were extracted by ethanol as solvent reflux

The total flavonoids were determined by spectrophotometry

rutin as control sample.Result:The absorbency and concentration of rutin have good linear correlation in the range of 5-30 μg·L-1.The regression equation was Y=0.011 2X+0.041 9（r=0.999 1）

the average recovery rate was 98.56%

Accuracy experiment RSD 0.279%

repeatability test RSD 0.634%

stability test RSD 1.49%

the contents of total flavonoids in leaf and stem was respectively 12.30% and 1.42%

that the means content of total flavonoids in leaf is 8.7 times higher than that in stem.Conclusion: the content of total flavonoids in the leaf and in the stem has a big difference

thereby the leaf of B.lineate from Duyun in Guizhou has value to be developed and used.

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