Simultaneous Determination of 7 Alkaloids in Zuojin Pill Ethanol Extracts by HPLC Coupled with Switching Wavelength

DING Shi-lan1; CUI Yuan-lu1; ZHANG Ye1; WANG Qiang-song1; DONG Tian-jiao1

doi:10.13422/j.cnki.syfjx.2012.15.032

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Simultaneous Determination of 7 Alkaloids in Zuojin Pill Ethanol Extracts by HPLC Coupled with Switching Wavelength

更新时间：2024-09-23

- Simultaneous Determination of 7 Alkaloids in Zuojin Pill Ethanol Extracts by HPLC Coupled with Switching Wavelength
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 15, Pages: 66-70(2012)
- 作者机构：
  
  1. 天津市现代中药重点实验室
  2. 2. 天津中医药大学中医药研究院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.15.032
  CLC： R284.1
- Published：2012
- 稿件说明：
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[1]丁世兰,崔元璐,张叶,王强松,董天骄.切换波长HPLC同时测定左金丸乙醇提取物中7种生物碱的含量[J].中国实验方剂学杂志,2012,18(15):66-70.

DING Shi-lan1, CUI Yuan-lu1, ZHANG Ye1, et al. Simultaneous Determination of 7 Alkaloids in Zuojin Pill Ethanol Extracts by HPLC Coupled with Switching Wavelength[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(15): 66-70.
[1]丁世兰,崔元璐,张叶,王强松,董天骄.切换波长HPLC同时测定左金丸乙醇提取物中7种生物碱的含量[J].中国实验方剂学杂志,2012,18(15):66-70. DOI： 10.13422/j.cnki.syfjx.2012.15.032.

DING Shi-lan1, CUI Yuan-lu1, ZHANG Ye1, et al. Simultaneous Determination of 7 Alkaloids in Zuojin Pill Ethanol Extracts by HPLC Coupled with Switching Wavelength[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(15): 66-70. DOI： 10.13422/j.cnki.syfjx.2012.15.032.

摘要

目的:建立同时测定左金丸乙醇提取物中表小檗碱、盐酸药根碱、黄连碱、盐酸巴马汀、盐酸小檗碱、吴茱萸碱和吴茱萸次碱含量的液相色谱法。方法:采用Kromasil C18（4.6 mm×250 mm

5μm）柱

以乙腈-0.1%磷酸和0.05%三乙胺水溶液为流动相进行梯度洗脱

检测波长0～40 min为265 nm

40～55 min为225 nm。结果:表小檗碱、盐酸药根碱、黄连碱、盐酸巴马汀、盐酸小檗碱、吴茱萸碱、吴茱萸次碱的线性范围分别是0.275 9～17.66

0.267 0～17.09

0.262 5～16.80

0.221 4～14.17

0.948 7～60.71

0.050 4～3.229

0.044 9～2.874 mg·L-1

平均回收率分别为100.4%

99.8%

100.2%

100.0%

99.0%

98.9%

97.4%

RSD分别为1.8%

1.2%

2.1%

2.0%

2.2%。结论:本法简便、准确

可同时测定左金丸乙醇提取物中表小檗碱、盐酸药根碱、黄连碱、盐酸巴马汀、盐酸小檗碱、吴茱萸碱和吴茱萸次碱的含量

为左金丸的现代制剂研究和质量控制提供方法学参考。

Abstract

Objective: To simultaneously determine epiberberine

jatrorrhizine

coptisine

palmatine

berberine

evodiamine and rutaecarpine in Zuojin pill ethanol extracts.Method: The chromatographic analysis was carried out on a Kromasil C18 column（4.6 mm×250 mm

5 μm）.The mobile phase comprised acetonitrile（A） and water phase（B） [0.1% phosphoric acid and 0.05% triethylamine].Detection was performed at a wavelength of 265 nm（0-40 min） and 225 nm（40-55 min）.Result: Epiberberine

jatrorrhizine

coptisine

palmatine

berberine

evodiamine and rutaecarpine had a good linearity in the range of 0.275 9-17.66

0.267 0-17.09

0.262 5-16.80

0.221 4-14.17

0.948 7-60.71

0.050 4-3.229 and 0.044 9-2.874 mg·L-1.The average recovery was 100.4% with RSD of 1.8%

99.8% with RSD of 1.2%

100.2% with RSD of 2.1%

100.0% with RSD of 2.1%

99.0% with RSD of 2.0%

98.9% with RSD of 2.0% and 97.4% with RSD of 2.2%.Conclusion: This is a simple

accurate method for determining the content of epiberberine

jatrorrhizine

coptisine

palmatine

berberine

evodiamine and rutaecarpine in Zuojin pill ethanol extracts.

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Related Author

DING Shi-lan

CUI Yuan-lu

ZHANG Ye

WANG Qiang-song

DONG Tian-jiao

TIAN Jun

HE Fei

LI Liu-hui

Related Institution

Beijing Key Laboratory for Quality Evaluation of Chinese Materia Medical/Traditional Chinese Medicine（TCM） Processing Technology Inheritance Base of National Administration of TCM， School of Chinese Materia Medica， Beijing University of Chinese Medicine

Traditional Chinese Medicine （TCM） Regulating Metabolic Diseases Key Laboratory of Sichuan Province， Hospital of Chengdu University of TCM

Cancer Institute， Chengdu University of TCM

China State Institute of Pharmaceutical Industry

Shanghai Institute of Food and Drug Control

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