Determination of Flavonoid of Bupleurum marginatum by HPLC

LIN Hai-xia1; WANG Shu-lin2; WANG Yan1; SUN Cui-ping1

doi:10.13422/j.cnki.syfjx.2012.15.034

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Determination of Flavonoid of Bupleurum marginatum by HPLC

更新时间：2024-09-23

- Determination of Flavonoid of Bupleurum marginatum by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 15, Pages: 76-79(2012)
- 作者机构：
  
  1. 成都中医药大学
  2. 成都中医药大学峨眉学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.15.034
  CLC： R284.1
- Published：2012
- 稿件说明：
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[1]林海霞,王书林,王砚,孙翠萍.HPLC测定竹叶柴胡中黄酮类成分的含量[J].中国实验方剂学杂志,2012,18(15):76-79.

LIN Hai-xia1, WANG Shu-lin2, WANG Yan1, et al. Determination of Flavonoid of Bupleurum marginatum by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(15): 76-79.
[1]林海霞,王书林,王砚,孙翠萍.HPLC测定竹叶柴胡中黄酮类成分的含量[J].中国实验方剂学杂志,2012,18(15):76-79. DOI： 10.13422/j.cnki.syfjx.2012.15.034.

LIN Hai-xia1, WANG Shu-lin2, WANG Yan1, et al. Determination of Flavonoid of Bupleurum marginatum by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(15): 76-79. DOI： 10.13422/j.cnki.syfjx.2012.15.034.

摘要

目的:建立竹叶柴胡中黄酮类成分（芦丁、槲皮素、山奈素和异鼠李素）HPLC的含量测定方法

为该药材的开发利用提供科学依据。方法:采用C18柱

流动相为甲醇（A）-0.1%磷酸（B）梯度洗脱

流速0.8 mL·min-1

检测波长360 nm

柱温35℃

进样体积10μL。结果:芦丁在667.8～33 390 ng（r=0.999 8）

槲皮素在216.0～10 798.4 ng（r=0.999 9）

山奈素在27.5～1 372.8 ng（r=0.999 9）

异鼠李素在130.6～6 528.0 ng（r=0.999 8）线性关系良好

平均加样回收率分别为100.3%（RSD 0.78%）

94.5%（RSD 1.13%）

94.5%（RSD 1.15%）

94.1%（RSD 1.09%）。结论:该法在同一色谱系统中同时测定芦丁、槲皮素、山奈素和异鼠李素的含量

操作简便、准确性高、重复性好

用于竹叶柴胡药材的质量评价。

Abstract

Objective:To develop a method by HPLC for determination of flavonoid of Bupleurum marginatum.Method: Agilent Zorbax SB C18 column（4.60 mm×150 mm

5 μm）

mobile phase methanol-0.1% phosphoric gradient elution

flow rate 0.8 min·mL-1

detected wavelength 360 nm.Result:The linear ranges of rutin

quercetin

kaempferol and isorhamnetin were 667.8-33 390 ng（r=0.999 8）

216.0-10 798.4 ng（r=0.999 9）

27.5-1 372.8 ng（r=0.999 9）

and 130.6-6 528.0 ng（r=0.999 8）

the average recovery rates were 100.3%（RSD 0.78%）

94.5%（RSD 1.13%）

94.5%（RSD 1.15%） and 94.1%（RSD 1.09%）.Conclusion:The HPLC method is simply and rapid

and suitable to assess qualitative of B.marginatum.

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Related Institution

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