Determination of Oleanolic Acid and Ursolic Acid Content in Dracocephalum moldovia by RP-HPLC

Ali·Haibuliqiemu; LI Gui-rong; YAN Bo; ZHANG Hao-ke

doi:10.13422/j.cnki.syfjx.2012.15.035

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Determination of Oleanolic Acid and Ursolic Acid Content in Dracocephalum moldovia by RP-HPLC

更新时间：2024-09-23

- Determination of Oleanolic Acid and Ursolic Acid Content in Dracocephalum moldovia by RP-HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 15, Pages: 80-82(2012)
- 作者机构：
  
  新疆医科大学第一附属医院药学部
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.15.035
  CLC： R284.1
- Published：2012
- 稿件说明：
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[1]布海力且木·阿里,李桂荣,闫波,张浩科.HPLC测定香青兰中齐墩果酸和熊果酸的含量[J].中国实验方剂学杂志,2012,18(15):80-82.

Ali·Haibuliqiemu, LI Gui-rong, YAN Bo, et al. Determination of Oleanolic Acid and Ursolic Acid Content in Dracocephalum moldovia by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(15): 80-82.
[1]布海力且木·阿里,李桂荣,闫波,张浩科.HPLC测定香青兰中齐墩果酸和熊果酸的含量[J].中国实验方剂学杂志,2012,18(15):80-82. DOI： 10.13422/j.cnki.syfjx.2012.15.035.

Ali·Haibuliqiemu, LI Gui-rong, YAN Bo, et al. Determination of Oleanolic Acid and Ursolic Acid Content in Dracocephalum moldovia by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(15): 80-82. DOI： 10.13422/j.cnki.syfjx.2012.15.035.

摘要

目的:采用反相高效液相色谱法测定香青兰中齐墩果酸和熊果酸的含量。方法:采用Kromasil C18柱（4.6 mm×250 mm

5μm）

流动相甲醇-0.2%磷酸二氢钠（88∶12）

流速1.0 mL·min-1

检测波长210 nm

柱温25℃。结果:齐墩果酸与熊果酸线性范围分别为0.42～2.10μg（r=0.999 4）

0.436～2.18μg（r=0.999 7）;平均回收率分别为齐墩果酸98.9%

RSD 1.14%（n=6）;熊果酸98.1%

RSD 1.80%（n=6）。结论:该方法快速、准确、可靠

可用于同时测定香青兰中齐墩果酸和熊果酸的含量。

Abstract

Objective: Use the method of HPLC to determine the conten of oleanolic acid and ursolic acid in Dracocephalum moldovia.Method: The content of oleanolic acid and ursolic acid was detected by HPLC.The separation was carried out on a Kromasil C18 column（4.6 mm×250 mm

5 μm）.The mobile phase was methanol-0.2% sodium dihydrogen phosphate（88∶ 12）;the flow rate was 1.0 mL·min-1;the detective wavelength was set at 210 nm.The column temperature was at 25 ℃.Result: The linear ranges of oleanolic acid and ursolic acid were 0.42-2.10 μg（r=0.999 4） and 0.436-2.18 μg（r=0.999 7）

respectively.The average recoveries were 98.9% with RSD of 1.14%（n=6） and 98.1% with RSD of 1.80%（n=6）

respectively.Conclusion: This method was rapid

accurate and reliable;it could be used to determine the content of oleanolic acid and ursolic acid in D.moldovia simultaneously.

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