LIU Zhi1, WANG Ai-mim2, XU Zu-chao3, et al. Study on HPLC Fingerprint of Tall Gastrodia Capsules[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(15): 86-88.
LIU Zhi1, WANG Ai-mim2, XU Zu-chao3, et al. Study on HPLC Fingerprint of Tall Gastrodia Capsules[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(15): 86-88. DOI: 10.13422/j.cnki.syfjx.2012.15.036.
Study on HPLC Fingerprint of Tall Gastrodia Capsules
摘要
目的:建立全天麻胶囊的HPLC特征指纹图谱分析方法
完善其质量控制方法
为评价全天麻胶囊的质量提供依据。方法:采用Phenomenex Hydro-RP 80A(4.6 mm×150 mm
4μm)色谱柱;流动相乙腈-0.1%磷酸溶液
梯度洗脱
流速1mL·min-1
柱温40℃
检测波长270 nm。结果:建立了全天麻胶囊的HPLC特征指纹图谱共有模式
标定了33个共有锋
指认了其中的8个共有锋
25批次全天麻胶囊的相似度为0.924~0.990。结论:该方法简单
准确
重复性好
可用于评价全天麻胶囊的质量。
Abstract
Objective: To study the HPLC fingerprint of tall gastrodia capsules to provide the reliable method for its quality control.Method: The separation was performed by a Phenomenex Hydro-RP 80A column(4.6 mm×150 mm
4 μm)with a mobile phase composed of acetonitrile-0.1% phosphonic acid using gradient elution at a flow rate of 1 mL·min-1.The column was maintained at 40 ℃ and wavelength detection was set at 270 nm.Result: HPLC fingerprint of Tall gastrodia capsules from 25 batches contained 33 common peaks
and eight peaks in the fingerprint were identified
the similarities ranged from 0.924 to 0.990.Conclusion: This method was simple
accurate with good reproducibility
which can be used specifically for the quality control of tall gastrodia capsules.